ac09电化学检测凝血酶固态适体探针.pdfVIP

Anal. Chem. 2010, 82, 1556–1563 Solid-State Probe Based Electrochemical Aptasensor for Cocaine: A Potentially Convenient, Sensitive, Repeatable, and Integrated Sensing Platform for Drugs Yan Du,†,‡ Chaogui Chen,†,‡ Jianyuan Yin,§ Bingling Li,†,‡ Ming Zhou,†,‡ Shaojun Dong,*,† and Erkang Wang*,† State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P. R. China, Graduate School of the Chinese Academy of Sciences, Beijing 100039, P. R. China, and Department of Nature Product Chemistry, Pharmacy College, Jilin University, Changchun, Jilin 130021, P. R. China Aptamers, which are artificial oligonucleotides selected to specific targets.4-7 Due to the inherent selectivity, affinity, and in vitro, have been employed to design novel biosensors multifarious advantages over the traditional recognition elements, (i.e., aptasensors). In this work, we first constructed a aptamers have attracted more and more attention and have been label-free electrochemical aptasensor introducing a probe widely developed in many research fields. Especially, label-free immobilization technique by the use of a layer-by-layer aptamer-based biosensors (i.e., aptasensors) play an important role (LBL) self-assembled multilayer with ferrocene-appended for their simplicity, convenience, and low cost. poly(ethyleneimine) (Fc-PEI) on an indium tin oxide Recently, a lot of label-free electrochemical aptasensors have (ITO) array electrode for detection of cocaine. The Fc-PEI been developed, and the electrochemical method mainly included and gold nanoparticles (AuNPs) were LBL assembled on electrochemical impedance spectroscopy (EIS),8-11