丝氨酸蛋白酶研究进展_汪世华.pdfVIP

福建农业学报22(4):453～456,2007 :1008-0384 (2007)04-0453-04 F uj ian Journal of Agricultural Sciences 汪世华, 王文勇, 黄益洲, 林　琳, 沙　莉 (, 　　350002) 　:、 、 。 , , 。 Ser、 His、 Asp, 。 , 。, 。 :;;; :Q 55 :A On serine protease ANG Shi-hua, ANG en-yong, HUANG Yi-zhou, LIN Lin, SHA Li (College of Lif eScience, F uj ian Agriculture and Forestry University, F uzhou, Fuj ian, 350002, China) Abstract:This paper reviews the serine proteases structures, functions, protein engineering, expression system, and applications.Serine protease isa group of proteolyticenzymes that are important for its potential applications. All serine proteaseshave serine, histidine and aspartic acid in their active domain, and same catalytic mechanism. The membersin the serine protease super family serve a wide range of physiological functions, especially in the pathogenesisof many diseases and intracellular signal transduction.Any minute alternationsin their structures can result in significant functional changes. Key words:serine protease;structure;function;application 　　 1.1　 , β, [1-2] , (N C ) 。 [5] 。, 、 、 、 。 [3-4] , 。 (Asp 102 His 57)N , Ser 195C , 1　丝氨酸蛋白酶的结构与功能 C , , (Gly 193 Ser 195), Ser、 His、 Asp, (Ser 189、Gly 216 Gly 226), , (Ser 214, Trp 215 Gly 。, [6] 216) 。C , , C , 。 C