油用牡丹凤丹白中SAD基因的克隆.docVIP

油用牡丹“凤丹白”中SAD基因的克隆 摘 要 牡丹种子产量高油中含有大量的不饱和脂肪酸（90%），其中油酸约占ω-6脂肪酸α-亚麻酸（ω-3脂肪酸）高达4%。牡丹籽油可以作为一种可持续利用的食用油。尽管如此，大多数的牡丹研究者都致力于牡丹的花芽发育、休眠、二次开花以及切花保鲜等研究，而对不饱和脂肪酸合成机制的研究才刚刚起步。油用牡丹的优良品种凤丹白生长繁殖快且种子含油率和亚麻酸含量均较高因此本以牡丹凤丹SAD基因，克隆 Cloning of SAD gene from peony Feng Dan Bai in oil ABSTRACT Peony high seed yield, oil content of up to 27% and oil containing a large amount of unsaturated fatty acids (90%), which accounts for about 21% of oleic acid, linoleic acid (omega-6 fatty acid) accounted for about 26%, alpha linolenic acid (omega-3 fatty acids) as high as 45%. Peony seed oil can be used as a new resource for sustainable utilization of edible oil. In spite of this, most of the peony researchers are devoted to the study of the flower bud development, dormancy, the two flowering and the preservation of cut flowers, and the study on the mechanism of the synthesis of unsaturated fatty acids is just started. Oil with fine varieties of peony Feng Dan Bai fast growth and reproduction and seed oil content and linolenic acid content were higher. Therefore, the research to peony Feng Dan Bai as the material extracted RNA of peony seeds at different developmental stages, the DNase digested cDNA was synthesized by reverse transcription. According to the report of the tree peony seed transcription group, the primers were designed, the SAD gene was cloned and sequenced and identified with the seed cDNA as the template. The research results of this paper will lay the foundation for the study of the molecular mechanism of the synthesis of unsaturated acid in oil. KEY WORDS: Oil With Peony， Feng Dan Bai， SAD gene， Clone目　录 前　言 1 第1章 综述 2 1.1 油用牡丹“凤丹白”的概述 2 1.1.1 油用牡丹（Paeonia suffruticosa Andr） 2 1.1.2 凤丹白 2 1.1.3 牡丹籽油 3 1.1.4 牡丹籽油提取工艺的研究 3 1.1.5 油用牡丹的产业发展 4 1.2 脂肪酸的简介 4 1.2.1 脂肪酸 4 1.2.2 脂肪酸的生理功能 5 1.2.3 多不饱和脂肪酸 5 1.2.4 多不饱和脂肪酸的来源 6 1.3 植物中脂肪酸的合成途径 6 1.4 脂肪酸脱氢酶 8 1.5 脂肪酸脱氢酶基因的研究现状 9 第2章 实验材料与方法 10 2.1 实验材料 10 2.1.1 菌株和载体 10 2.1.2 植物材料 10 2.1.3 试剂和药品 10 2.1.4 仪器和设备 12 2.1.5 培养基及溶液 13 2.2 实验方