B16_F10_luc_G5黑色素瘤细胞生物学特性研究.pdf

2013 10 21 10 MODEＲN ONCOLOGY ，Oct. 2013 ，VOL. 21，NO. 10 ·2 169 · 现代肿瘤医学 年 月 第 卷第 期 B16 － F10 － luc － G5 黑色素瘤细胞生物学特性的研究 ， ， 苏云术 周鸿敏 徐利军 Cytobiological characters of mouse melanoma cell lines B16 － F10 － luc － G5 Su Yunshu ，Zhou Hongmin ，Xu Lijun Dep artment of Cardiothroracic Surgery ，Tongj i Hosp ital ，Huazhong University of Science and Technology ，Hubei Wuhan 430030 ，China． 【Abstract 】 Objective: To study the biological characteristics of B16 － F10 mouse melanoma cell labeling luc gene ( B16 － F10 － luc － G5) ． Methods: Tumor dynamic morphology of B16 － F10 － luc － G5 was observed under fluores- cence microscope． Activity after various kinds of experimental treatment was detected by flow cytometry． B16 － F10 － luc － G5 cells were diluted from 10 ，000 cells to 78 cells and then the cell plate was imaged using IVIS system to eval- uate its luminescence characteristics． Ten Babl / C and C57bl / 6 mice with subcutaneously injected tumor cells ( 1 × 105 in 100 μl) ，then the growth curves and pathologic changes of transplanted tumor were compared． Luminescences of transplanted tumor were sequentially detected by IVIS． Ｒesults: Tumor dynamic morphology of B16 － F10 － luc － G5 was nearly the same as typical B16 － F10 cell line． Apoptosis of B 16 － F 10 － luc － G5 cells under several condi- tions weredetected by flow cytometry ． A strong correlation between number of tumor cells and bioluminescence ( r = 0 ． 998) was also demonstrated by IVIS． There was significant difference ( P ＜ 0 ． 01) of tumor growth between two groups． Tumor dynamic morphology could still be detected by IVIS even when its cell was invisible． Conclusion: B16 － F10 － luc － G5 cell labeled with luc gene ，had typical biological characteristics