抗莠去津抗体基因构建及蛋白结构模拟 - 植物生态学报.pdf

※生物工程 食品科学 2015, Vol.36, No.23 195 抗莠去津抗体基因构建及蛋白结构模拟 * 东 琴，魏松红 ，李平生，王海宁 （沈阳农业大学植物保护学院，辽宁沈阳 110866 ） 摘  要：构建抗莠去津单链抗体基因，采用DNAStar软件对单链抗体基因进行氨基酸序列推导，用生物信息学在线 网站预测莠去津单链抗体基本特性及二、三级结构。结果表明，抗莠去津单链抗体基因全长744 bp ，对应248 个氨 α 基酸，单链抗体相对分子质量约为26 061.9 ，等电点预测值7.81 ，为碱性蛋白质；二级结构中 -螺旋17 处，延伸链 β 85 处， -转角19 处，无规卷曲127 处；三级结构建模显示，重链和轻链的6 个环区共同组成了抗体的抗原结合区， 符合单链抗体的结构特点，具有抗原结合位点的空间构象。该研究为抗莠去津单链抗体的进一步表达、纯化和活性 研究奠定了基础。 关键词：莠去津；单链抗体；结构预测 Gene Construction and Protein Structure Prediction of Single-Chain Variable Fragment Antibody against Atrazine * DONG Qin, WEI Songhong , LI Pingsheng, WANG Haining (Plant Protection College, Shenyang Agricultural University, Shenyang 110866, China) Abstract: In order to obtain the gene encoding single-chain variable fragment (scFv) antibody against atrazine, the amino acid sequence of scFv was deduced by DNAStar software and its physical and chemical properties, and secondary and tertiary structures of its encoding protein were analyzed according to bioinformatics online websites. The results showed that the full-length scFv gene had 744 bp and encoded 248 amino acids. The relative molecular weight of scFv was 26 061.9, and the antibody had isoelectric point (pI) of 7.81 and belonged to alkaline and stable protein. The predicted secondary structure comprised 17 α-helices, 85 extended strands, 19 β-turns and 127 random coils. The mimic tertiary structure model of scFv indicted that all six complementarity-determining regions (CDRs) of light chain variable (VL) and heavy chain vari