槲皮素对大鼠原代肝细胞ho-1 蛋白表达及活性的影响.pdf

营养学报2011 年第33卷第 6 期 555 槲皮素对大鼠原代肝细胞 HO-1 蛋白表达及 活性的影响    关键词： 槲皮素； 肝细胞； I 型血红素氧化酶 中图分类号：R141.1 文献标识码：A 文章编号：0512-7955（2011）06-0555-04 EFFECTS OF QUERCETIN ON HEME OXYGENASE-1 PROTEIN EXPRESSION AND ENZYME ACTIVITY IN RAT PRIMARY HEPATOCYTES LIU Shuang ，YAO Ping ，GONG Chen-rui ，LI Jun ，CHENG Mao-wei ，LIU Lie-gang 1 2 ( Department of Nutrition and Food Hygiene, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030 ； Hubei Center for Disease Control and Prevention , Wuhan 430079, China) 【Abstract 】Objective To investigate the effects of quercetin on heme oxygenase-1 (HO-1) protein expression and enzyme activity in rat primary hepatocytes. Method Sprague-Dawley rat primary hepatocytes were isolated using two-step collagenase perfusion technique. The hepatocytes were treated with quercetin of different doses (25 -200 μmol/L) for 12 h, or were treated with 50 μmol/L quercetin for different time (4 -12 h). Then Western blot and enzymatic assays were deployded to determine the protein expression and the enzyme activity of HO-1 in hepatocellular microsomes. Results After co-incubation with quercetin at the dose of 50 -200 μmol/L for 4 -12 h, the protein level and the enzyme activity of HO-1 in hepat