高敏CRP、vWF和CD62P联合检测在冠心病患者的应用.pdfVIP

136 Labeled Imrnunoassays&Clin Med， Jun．2008，VoL 15，NO．3 高敏CRP、vWF和CD62P联合检测 在冠心病患者的应用 高淑芳，姜维洁，董静懿 (上海中医药大学附属龙华医院检验科，上海 200032) 摘要：探讨超敏c反应蛋白(hs—CRP)、血管性血友病因子(von Willebrand factor，vWF)、血小板表面P选择素 (CD62P)的变化与冠心病患者的关系及其临床价值。对 4O例冠心病患者(不稳定性心绞痛)及3O名正常人采用 乳胶增强免疫比浊法测定hs—CRP，ELISA测血浆vWF，流式细胞仪(FCM)测定血小板膜CD62P阳性百分率 ( )。结果显示，冠心病组的hs—CRP显著高于对照组(P<0．01)，血浆vWF显著高于对照组(P<O．01)，CD62P 阳性率显著高于对照组(P<O．01)。联合检测hs—CRP、vWF和CD62P对冠心病患者的辅助诊断、疗效和预后观 察有一定价值。 关键词：高敏c反应蛋白； 血管性血友病因子；血小板表面P选择素； 冠心病 中图分类号：R 541．4 文献标识码：A 文章编号：1006—1703(2008)03—0136—03 Application of Combined Detection of hs—CRP，vWF and CD62P in Assessment of Patients with Coronary Heart Disease GAO Shu—fang，JIANG Wei_j ie，DONG Jing—yi (Clinical Laboratory，the Affiliated Long Hua Hospital of Shanghai Traditional Medicine University，Shanghai 200032，China) Abstract：To explore the changes and clinical value of serum hs—CRP，vWF and CD62P levels in patients with coronary heart disease。the serum hs—CRP level was measured by latex enhanced immunoturbidimetry．the plasma vWF level was detected by ELISA and the percentage of CD62P in citrated whole blood was detected by flow cytometry in 40 patients with coronary heart disease(unstable angina)and 30 health controls．The results showed that the serum le— vels of hs—CRP and vWF in patients with coronary heart disease were higher than those in the healthy controls(P<0．01)．The percentage of CD62P in patients with coronary heart disease was higher than that in the healthy group(P<0．01)．These data suggest that combined detec— tion of hs—CRP．vWF and CD62P levels in patients with coronary disease might have clinical significance in the suppl