响应面设计优化绿僵菌固体发酵条件.pdf

微生物学通报 Sep. 20, 2016, 43(9): 2072−2078 Microbiology China /wswxtbcn tongbao@ DOI: 10.13344/j.microbiol.china.150817 简 报 响应面设计优化绿僵菌固体发酵条件 张婧迪 蔡明美 刘志诚 陈捷* ( 200240) 摘 要：【目的】为了提高绿僵菌分生孢子产量及孢子质量，应用响应面设计对金龟子绿僵菌菌 株CY-1 (Metarhizium anisopliae)进行固体发酵培养基的优化。【方法】单因素试验基础上，采用 响应面试验设计方法优化培养基组分。【结果】添加了碳、氮营养的最佳固体发酵培养基为玉米 粉:稻壳=8:2 ，料水比1:0.8，葡萄糖0.8% ，硫酸铵2.5% ，磷酸二氢钾0.8%；在固体培养基上的 理论产孢量为 7.45×109 个/g ；验证后实际为 6.94×109 个/g 。【结论】运用响应面法对绿僵菌固体 发酵的培养基成分进行优化，得到了绿僵菌孢子粉，为孢子粉进行地下害虫防治和制剂加工的研 究奠定了基础。 关键词：绿僵菌，响应面法，优化，固体发酵 Metarhizium solid-state fermentation optimization by response surface analysis * ZHANG Jing-Di CAI Ming-Mei LIU Zhi-Cheng CHEN Jie (College of Agriculture and Biology, Shanghai Jiao Tong University, Ministry of Agriculture Key Laboratory of Urban Agriculture in the South, State Key Laboratory of Microbial Metabolism, Shanghai 200240, China) Abstract: [Objective] In order to improve the yield and quality of the Metarhizium conidia spores, response surface design was used to optimize the medium components for solid fermentation of the Metarhizium anisopliae CY-1. [Methods] On the basis of one-factor experiment, medium components were optimized by the response surface design. [Results] The carbon and nitrogen sources were added to the best solid fermentation medium, which also contained cornmeal and rice husk (8:2, W/W), water ratio 1:0.8, 0.8%