gankyrin蛋白在大鼠肝细胞癌变过程中的异常表达 - 第三军医大学学报.doc

Gankyrin蛋白在大鼠肝细胞癌变过程中的异常表达及意义 韩克强1，吴娅利2，黄小兵1，李 靖1，梁 平1，郑 璐1 （第三军医大学：1新桥医院肝胆外科，重庆 400037；2，重庆 4000）Gankyrin蛋白的异常表达及其意义。方法 用0.2%二乙基亚硝胺(diethylnitrosamine，DENWistar大鼠建立肝癌动物模型，应用免疫组化法检测大鼠肝脏在癌变及癌转移过程中Gankyrin蛋白的表达。结果 20例正常组中未见Gankyrin蛋白表达，21例肝硬化组中有3例Gankyrin蛋白过表达，68例肝癌组（未转移）中有46例Gankyrin蛋白过表达，18例肝癌组（伴转移）中有17例Gankyrin蛋白过表达。结论 Gankyrin蛋白的阳性表达率随病变程度的加重而逐渐升高，这提示Gankyrin蛋白在肝细胞癌形成过程中可能发挥重要的促癌作用，并可能促进了肝癌的转移和高侵袭性发展，其有可能成为肝细胞癌转移早期监测的参考指标。 关键词：肝细胞癌;Gankyrin蛋白;免疫组化;大鼠 Abnormal expression of Gankyrin protein during hepatocarcinogenesis in rats and its significance HAN Ke-qiang1, WU Ya-li2, HUANG Xiao-bing1, LI Jing1, LIANG Ping1, ZHENG Lu1 (1Department of hepatobiliary surgery, Xinqiao Hospital, Chongqing 400037, 2Office of Clinical Management, Training Department, Third Military Medical University, Chongqing 400038, China) Abstract: Objective To investigate the abnormal expression of Gankyrin protein during hepatocarcinogenesis in rats and its significance. Methods DEN solution was administered in Wistar rats to induce hepatocellular carcinoma(HCC), then the expression of Gankyrin protein was detected by immunohistochemistry during hepatocarcinogenesis. Results No expression of Gankyrin protein was found in the 20 cases of control group. Gankyrin protein overexpression was found 3 cases out of 21 cases of liver cirrhosis, 46 cases out of 68 cases of HCC(without metastasis), 17 cases out of 18 cases of HCC(with metastasis). Conclusion With the progression of hepatocarcinogenesis, the positive rates of Gankyrin protein expression is more high. Which means Gankyrin protein could promote hepatocarcinogenesis, and also play a major role in the invasion and metastasis of HCC. Gankyrin protein may be an effective referenced index for early monitoring for metastasis in HCC. Key words: hepatocelluar carcinoma; Gankyrin protein; immunohistochemistry; rat 肝癌发生的主要外因是肝炎病毒感染和肝特异性致癌物，同时肝癌和其它恶性肿瘤一样，其发生、发展、转移是由体内多基因参与、多步骤协同的复杂过程[1-3]。目前尽管已经鉴定了一些肝癌的抑癌基因，如p53和p16等，并明确了它们在肝癌中的特异性失活[4,5]，但