马氏珠母贝血清抗菌肽的初步分析.pdf

19 卷 5 期 生命科学研究 灾燥造援员9 晕燥援5 圆园15 年 10 月 蕴蚤枣藻 杂糟蚤藻灶糟藻 砸藻泽藻葬则糟澡 Oct. 圆园15 马氏珠母贝血清抗菌肽的初步分析 朱家萍, 雷倩楠, 梁海鹰* (广东海洋大学水产学院, 中 广东湛江524088) 摘 要: 马氏珠母贝(Pinctada martens ii)是生产海水珍珠的主要经济贝类, 近年来马氏珠母贝因病害频繁发生 而对我国珍珠产业造成巨大的经济损失 因此, 对于马氏珠母贝的免疫机制研究尤为重要 抗菌肽是贝类生物 免疫机制中最重要的一个环节, 但目前对马氏珠母贝抗菌肽的报道甚少 为深入了解马氏珠母贝的抗菌肽分 子组成及特征, 本实验采取多步高效液相色谱结合质谱分析, 对马氏珠母贝血清中具有抗菌活性的蛋白质分 子开展了分离纯化及鉴定研究, 得到了5 种有明显抗菌活性的蛋白, 其中3 种相对分子质量在5 000 kD 左右; 通过透射电镜进一步观察了抗菌活性蛋白对细菌的作用机制 上述研究为了解马氏珠母贝抗菌肽的分子多样 性及抗菌机制奠定了基础 关键词: 马氏珠母贝; 抗菌肽; 免疫机制; 质谱; 抑菌实验 中图分类号: Q51 文献标识码: A 文章编号: 1007-7847(2015)05-0397-05 Analysis of Antimicrobial Peptides from Serum of Pinctada martensii ZHU Jia-ping, LEI Qian-nan, LIANG Hai-ying* (Fisheries College, Guangdong Ocean Unive rs ity, Zhanj iang 524088, Guangdong, China) Abstract: Pinctada martens ii is a major economic shellfish for sea pearls. Due to the diseases anually, a large of P. martens ii died every year and resulting in a huge economic losses on China ’s pearl industry. The immune system and the mechanism of P. martens ii against pathogenic bacteria is not clear so far. Antimicro bial peptide is one of the most important immue molecular for shellfish, however, little sequence and charac terization of antimicrobial peptides was reported from P. martens ii. In order to understand the protein com position and molecular diversity of P. martens ii antimicrobial peptide, multi -step high performance liquid chromatogram (HPLC) combined mass spectrometry were performed to exploring the peptides with antimicro bial activity. 5 components