酸化ストレスによる生体高分子の化学修饰机构 表皮ケラチン及び 升压 .pdf

YAKUGAKU ZASSHI 133(10) 1055―1063 (2013) 2013 The Pharmaceutical Society of Japan ―Review― 酸化ストレスによる生体高分子の化学修飾機構表皮ケラチン及び 昇圧ペプチド類の修飾解析とその生理的意義 李 宣和 Oxidative Stress-mediated Chemical Modiˆcations to Biomacromolecules: Mechanism and Implication of Modiˆcations to Human Skin Keratins and Angiotensin II Seon Hwa Lee Department of Bio-analytical Chemistry, Graduate School of Pharmaceutical Sciences,   Tohoku University; 6 3 Aoba, Aramaki, Aoba-ku, Sendai 980 8578, Japan. ( ) Received May 31, 2013 ( ) Dysregulated production of reactive oxygen species ROS during oxidative stress has been associated with a num- ber of in‰ammatory and age-related degenerative diseases. ROS can directly react with DNA to form oxidized DNA bases. Direct protein oxidation and carbonylation occur on certain amino acid residues resulting in various post-transla- tional modiˆcations. ROS can also initiate the formation of lipid hydroperoxides, which undergo homolytic decomposi- ( ) ( ) tion to the a,b-unsaturated aldehydic bifunctional electrophiles such as 4-oxo-2 E -nonenal ONE and 4-hydroxy-2 ( ) ( ) E -nonenal HNE . Intracellular generation of highly reactive aldehydes can then result in the formation of DNA and protein adducts. ONE-derived heptanone-etheno and HNE-derived pro