酶法提取马齿苋多糖 - 中国农学通报.pdf

中国农学通报 2011,27(05):475-478 Chinese Agricultural Science Bulletin 酶法提取马齿苋多糖 1 2 1 钱志伟 ，王会晓 ，杨海蛟 1 2 （河南农业职业学院，郑州451450 ；郑州轻工业学院食品与生物工程学院，郑州450002) 摘 要：为了优化马齿苋多糖的提取工艺，将纤维素酶用于提取马齿苋多糖，在单因素实验的基础上，采 4 用L (3 )正交实验法对酶法提取马齿苋多糖的条件进行优化，考察酶用量、酶解温度、pH 、酶解时间对马 9 齿苋多糖提取得率的影响。结果表明：最合适的工艺参数为酶用量2.0%、酶解温度40℃、pH 6.0、酶解时 间110 min 。该条件下，马齿苋多糖得率达到9.7% 。酶法提取纤维素多糖工艺简洁，能量消耗减少，是 一种提取马齿苋多糖的的适宜方法。 关键词：酶；提取；马齿苋；多糖 中图分类号：TS201.1 文献标志码：B 论文编号：2010-2281 EnzymeExtractionofPolysaccharidefromPortulacaoleraceaL. 1 2 1 Qian Zhiwei , Wang Huixiao , Yang Haijiao 1 ( HenanVocationalCollegeofAgriculture, Zhengzhou 451450; 2SchoolofFoodandBiologicalEngineering,ZhengzhouUniversityofLightIndustry, Zhengzhou 450002) Abstract:In order to extraction of polysaccharide from Portulaca oleracea L.. The optimum enzyme extraction 4 technology was optimized with L (3 ) orthogonal test. The influence of enzyme dosage,extraction temperature , 9 pH and extraction time on yield of polysaccharide was studied. The optimum conditions were as follows: dosage of cellulose 2.0%, temperature 40℃, time 110 min, pH 6.0 and extraction yield were 9.7%. It indicated that enzyme extraction was a suitable method for extraction polysaccharide from Portulaca oleracea L. for it was simple and has s