毛细管等电聚焦／加压毛细管电色谱二维分离体系 - researchgate.pdf

See discussions, stats, and author profiles for this publication at: /publication/267329257 Two-Dimensional Separation System by Coupling Capillary Isoelectric Focusing to Pressurized Capillary... Article in Journal of nalytical Chemistry · February 2011 DOI: 10.3724/SP.J.1096.2011.00188 CIT TIONS RE DS 0 45 4 authors, including: Juan Wei Yan Wang The University of Warwick Shanghai Jiao Tong University 16 PUBLIC TIONS 71 CIT TIONS 709 PUBLIC TIONS ,919 CIT TIONS SEE PROFILE SEE PROFILE Chao Yan Shanghai Jiao Tong University 276 PUBLIC TIONS 2,71 CIT TIONS SEE PROFILE Some of the authors of this publication are also working on these related projects: capillary electrochromatography View project NSCLC clinical and translational research View project ll content following this page was uploaded by Chao Yan on 08 July 2015. The user has requested enhancement of the downloaded file. ll in-text references underlined in blue are added to the original doc and are linked to publications on ResearchGate, letting you access and read them immediately. 第39卷 分析化学(FENXIHUAXUE)研究报告 第2期 of 2011年2月 ChineseJournal 188～192 AnalyticalChemistry 毛细管等电聚焦／加压毛细管电色谱二维分离体系 在多肽分离中的应用 魏娟 谷雪 王彦 阎超‘ (上海交通大学药学院，上海200240) 摘要 以毛细管等电聚焦(cIEF)为第一维分离模式，以反相加压毛细管电色谱(pCEC)为第二维分离模式， 开展离线二维色谱分离研究，并对复杂肽段进行分离。羟丙基纤维素(HPC)涂层的毛细管用于clEF分离，对 6种标准蛋白质的平均分离柱效约为31万。在毛细管末端引进电隔