链霉菌素复合物薄膜对麴霉菌和青霉菌之抗菌活性 - 宜兰大学食品科学系.DOC

國立宜蘭大學食品科學研究所 碩士班專題討論 演講者：邵郁涵 (Shao,Yu-han) 題目：鏈黴菌素複合物薄膜對麴黴菌和青黴菌之抗菌活性 指導教授： 陳輝煌 老師 Antifungal activity of films containing natamycin complex 林世斌 老師 against Aspergillus and Penicillum 日期：Dec.05, 2012 中文摘要 真菌生長在乳製品時，不僅會造成經濟損失，並且產生黴菌毒素導致健康問題。因此，本篇報告評估含有鏈黴菌素 (Natamycin, NA) 複合物的薄膜應用於乳酪包材時，對對微生物性質之影響。發現在10 g小麥蛋白薄膜 (wheat gluten film, WG film) 添加 2 mg 以上的鏈黴菌素 (NA)，在 10 ℃ 下儲存 30 天Aspergillus niger) 生長。10 g小麥蛋白薄膜 (WG film) 或甲基纖維素薄膜 (MC film) 複合 1.5mg 鏈黴菌素 (NA) 和1.5mL 迷迭香萃取物 (rosemary extract; RE)，具有協同效應，不僅能有效抑制黑麴菌 (Aspergillus niger)，亦降低鏈黴菌素(NA)的最低抑制濃度(minimum inhibitory concentrations; MIC)。另外，聚合物薄膜複合鏈黴菌素 (NA) 和乳酸鏈球菌素 (Nisin) 可有效抑制青黴菌(Penicillium expansum)生長。這些結果顯示，鏈黴菌素 (NA) 複合其他抗菌物質製成的薄膜具有抑制乳製品黴菌生長的潛力。 關鍵字：鏈黴菌素、麴黴菌、青黴菌、抗真菌薄膜 Abstract Fungal growth on dairy products not only causes economical losses but also results in health problems owing to mycotoxin production . Thus, the purpose of this study was to evaluate the effects of the application of polymer film containing natamycin complex on the microbial properties of cheese. The wheat gluten (WG) film containing natamycin (NA), above 2 mg per 10 g film, could completely inhibit Aspergillus niger during 30 day of storage at 10 ℃. Synergistic effect was observed when 1.5 mg natamycin (NA) and 1.5 mL rosemary extract (RE) were add to 10 g WG film or methyl cellulose (MC) film, which not only completely inhibited Aspergillus niger but also decrease the minimum inhibitory concentrations (MIC) value of natamycin (NA). Moreover, combination of natamycin (NA) and nisin in the coatings on polymer films inhibited the growth of Penicillium expansum.Therefore, films containing natamycin complex possesses potential ability to inhibit the fungal on dairy products. Keywords: Natamycin, Aspergillus nig