红外光谱技术在滇龙胆栽培方式选择上的应用 米丽菊1,2，张 霁1,3，左 .DOC

红外光谱技术在滇龙胆栽培方式选择上的应用 米丽菊1,2，张 霁1,3，左智天1,3，王元忠1,3*，李富生2 （1.云南省农业科学院药用植物研究所，昆明 650200；2.云南农业大学农学与生物技术学院，昆明 650201；3.云南省省级中药原料质量监测技术服务中心，昆明 650200） （Fourier transform infrared spectroscopy, FTIR）偏最小二乘判别分析（Partial least squares discriminant analysis, PLS-DA），能较好的区分不同栽培方式的滇龙胆样品，剪枝Orthogonal signal correction-partial least squares, OSC-PLS）回归模型预测不同栽培模式滇龙胆中龙胆苦苷、马钱苷酸、獐牙菜苦苷和当药苷的含量。校正集和验证集的决定系数（R2）均大于0.90，校正均方根误差（Root mean square error of estimation, RMSEE）、交叉验证均方差（Root mean squared error of cross-validation, RMSECV）和预测均方根误差（Root mean square error of prediction, RMSEP）均小于1.65，模型相关性和预测效果好，该法可对红外光谱分析在中药领域的推广应用提供参考。 关键词：傅里叶变换红外光谱，偏最小二乘判别分析，正交信号校正-偏最小二乘法，栽培方式，滇龙胆 中图分类号Q 文献标识A 文章编号DOI：10.11931/guihaia.gxzw201603012 Application of Fourier transform infrared spectroscopy selecting the cultivation method of Gentiana rigescens MI Li-Ju1,2, ZHANG Ji1,3, ZUO Zhi-Tian1,3, WANG Yuan-Zhong1,3*, LI Fu-Sheng2 (1. Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China; 2. College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China; 3. Yunnan Technical Center for Quality of Chinese Materia Medica, Kunming 650200, China) Abstract: Medicinal plants are the resources of traditional Chinese medicines (TCM), and selecting appropriate cultivation method is conductive to guarantee the quality of TCM from the source. In this research, in order to select optimal cultivation method for the Gentiana rigescens, Fourier transform infrared (FTIR) spectroscopy combined with chemometrics was used for analyzing the G. rigescens which were cultivated through sowing in drill, broadcast sowing, transplanting after root pruning, cutting and transplanting after pruning. The results showed that: (1) Original FTIR spectra of G. rigescens from different cultivation methods had some differences in shape, position and intensity of peak. After preprocessed with the wavelet denoising, the spectral data were analyzed by partial least squares d