呋喃丹对赤子爱胜蚓体内蛋白含量_SOD和TChE活性的影响_胡玲.pdfVIP

DOI :10.13989/j.cnki .0517 -6611.2006.13.112 , Journal of Anhui Agri.Sci.2006, 34(13):3165-3167　　　　　　　　　　　　　　　　　　　孙红忠　　孙红忠 、SOD TChE 1,2 2 ＊ 胡玲 , 林玉锁 　(1., 210095;2., , 210042) 　选用杀虫剂呋喃丹对赤子爱胜蚓(Eisenia f oetida)进行染毒试验, 研究农药污染对赤子爱胜蚓的 死率和体内蛋白含量、SOD 和 TChE 活力的影响。结果表明:当蚯蚓受到低浓度呋喃丹胁迫时, 其体内蛋白含量和TChE 的活性均表现为升高趋势, SOD 活性表现为降 低;当受到高浓度呋喃丹胁迫时, 其体内蛋白含量和TChE 活性表现为不同程度的降低, SOD 却表现为升高;其剂量-效应关系曲线均为 抛物线型。可利用蛋白含量、SOD 和TChE 活性的变化来指示呋喃丹对赤子爱胜蚓的毒性作用。 　呋喃丹;赤子爱胜蚓;蛋白含量;SOD;TChE 　Q599　　　A　　　0517-6611(2006)13-3165-03 Effect of Carbofuran on Protein Content and the SOD and TChE Activity of the Eisenia Foetida Earthworm HU Ling et a 　(College of Resources and En ironmental Science, Nanjing Agricultural Uni ersity, Nanjing,Jiangsu 210095) Abstract 　The insecticide carbofuran was selected to test the toxicity to Eisenia foetida.The pesticide pollution affects the death rate, protein content and SOD and TChE acti ity of theEisenia foetida earthworm.The results indicated that:with the carbofuran concentrations increasing, both the protein content and TChE acti ity in low le el were increased while decreased in high le el.The change of SOD acti ity of the trial animal wasnt appeared the similar rule.It s reduced when carbofuran concentration were increased and increased when carbofuran concentration arri ed at the lowest point.And dosage-ef- fect cur es all taken on parabola.Therefore the changes of protein content and SOD and TChE acti ity of the Eiseniafoetida earthworm can beused to indi- cate the effect of the insecticide carbofuran on the toxicity to Eisenia foetida earthworm. Key words　Carbofuran;Eisenia foetida earthworm;Protein content;SOD;TChE 　　(2,3--2,2--7--N- 1.4 　 ),1 1.4.1　。1L ～6 ,, ,( [1] ), 1 ( , 。 [4] (SOD), ) 。 , , 1.4.2 　。, 18 cm [2] [3-10] 18 cm 。 。 , ,