金顶侧耳不同生长期几种胞外酶活性变化 - 吉林农业大学学报.pdf

28 6 Vol28 No6 2006 12 Journal of Jilin Agricultural Univ rsity D c mb r 2006 1 2 1 3 李 娟 , 倪新江 , 樊守金 , 蔡德华 ( 1山东师范大学生命科学院, 济南250100; 2烟台大学生物化学系, 烟台264005; 3烟台 师范学院生命科学院, 烟台264025) : 用发酵的棉籽壳盆栽金顶侧耳, 测定其生长发育期间的 外酶活性变化结果表明: 金顶侧耳具有 完整的 外纤维素酶系, 外羧甲基纤维素酶( CMC 酶) 滤纸纤维素酶( FP 酶) 和半纤维素酶( HC 酶) 活性高 峰对应于子实体的生长发育期, 外蛋白酶和果胶酶活性在原基出现后升高并 一直维持在较高水平, 外淀 粉酶漆酶和过氧化物酶活性均随栽培时间的延长逐渐下降 : 金顶侧耳; 生长期; 外酶 : S64614 : A : 1000-5684( 2006) 06-0619-04 Activity Changes of Several Extracellular Enzymes in Different Growth Periods of Pleurotus citrinop ileat us 1 2 1 3 LI Jua n , NI Xin-jia ng , FAN Shou-jin , CA I De-hua (1 College of Lfie Science, Shandong Normal University , Jinan 250014, China; 2Department fo io- chemistry, Yantai University , Yantai 264005, China; 3 College of Lfie Science, Yantai Teachers Col- lege, Yantai 264025, China) Abstract: Activity chang s of s v ral xtrac llular nzym s w r d t rmin d in diff r nt growth p riods of Pleurotus citrinopileatus cultivat d on f rm nt d cotton s d hull compost in flow rpotTh r sults show d that th fungus had a compl t s t of xtrac llular c llulas Th activity p aks of xtrac llular CMCas ( carboxym nth l c llulas ) , FPas ( filt r pap r c llulas ) and HCas ( h mic llulas ) all app ar d in th p riod b tw n primordial formation and fruit-body maturity Th activity of xtrac llular prot as and p ctas incr as d st ply b tw n primordial formation p riod and fruit-body maturity p riod and it r -