进行性斑状色素减少症发病机制初步探讨.PDFVIP

·· ·· Chin J Dermato Venerol Integ Trad W Med 2012, Vol． 11 No．3 140 ·论著· 进行性斑状色素减少症发病机制初步探讨 李陶强，许爱娥，吴辛刚，尉晓冬 （安徽医科大学附属杭州临床学院，杭州 310009） 摘要：目的探讨进行性斑状色素减少症（progressive macular hypomelanosis, PMH）患者皮损中分离出的痤疮丙酸 杆菌对人角质形成细胞（human keratinocytes, HaCaT)细胞分泌干扰素（interferon, IFN）-γ、白细胞介素（interleukin, IL）- 6、肿瘤坏死因子（tumor necrosis factor, TNF）-α 的影响。方法体外培养HaCaT 细胞株，分别加入分离自PMH 的痤疮 丙酸杆菌、痤疮丙酸杆菌标准菌株（NCTC737），酶联免疫吸附试验（enzyme-linked immunosorbent assay, ELISA）方法 测定细胞上清液中IFN-γ、IL-6、TNF-α 的分泌量。结果 PMH 患者中分离的痤疮丙酸杆菌与角质形成细胞HaCaT 细 胞株共培养后，能促进角质形成细胞分泌IFN-γ 和IL-6 ，与空白组比较，有明显升高，差异有统计学意义（P＜0.05）； 与痤疮丙酸杆菌标准菌株NCTC737 相比也升高，差异有统计学意义（P＜0.05）。结论 进行性斑状色素减少症皮损中 分离出的痤疮丙酸杆菌可刺激HaCaT 细胞分泌IFN-γ、IL-6、TNF-α，提示可能是皮损色素减退的原因之一。 ： 关键词 丙酸杆菌；色素减退；细菌；细胞因子 ： （ ） 中图分类号：R758.4 文献标识码 A 文章编号：1672－0709 2012 03-0 140－03 Exploration of Pathogenesis of Progressive Macular Hypomelanosis LI Taoqiang, XU Ai ’e, WU Xingang, WEI Xiaodong Hangzhou Clinical College Affiliated to Anhui Medical University, Hangzhou 310009, China ： Abstract Objective To investigate the effects of Propionibacterium acnes from the lesions of progressive macular hypomelanosis （PMH）patients on the production of cytokines interferon （IFN）-γ, interleukin （IL）-6 and tumor necrosis factor （TNF）-α in human keratinocytes (HaCaT cells). Methods HaCaT cells were co-cultured with the isolates from the patients of PMH and NCTC 737 in vitro, respectively. IFN-γ, IL-6 and TNF-α were detected by enzyme-linked immunosorbent assay (ELISA) in the culture supernatants, which were harvested after 24 h of the HaCaT cells which co- cultured with the bacteria. Results The secretions of IFN -γ and IL -6 from the supernatants of PMH groups were significantly increased than that of others （P ＜0.