清营解表合剂治疗感冒养阴治法的实验探究.pdfVIP

提 要 目的：本文拟通过理论及实验研究，探讨解表为主，兼以滋阴治法在流感和某些 普通感冒中的应用，为临床更有效的防治感冒和提高临床疗效提供科学依据。 方法：本文通过回顾祖国医学对感冒病因病机和治法的认识，认为流感和某些普 通感冒在不同病理阶段出现阴伤症状。根据此病机特点，确立以“辛凉解表”为主， “滋阴凉营”为辅的治疗法则，制成清营解表合剂，方由银花、连翘、白薇、生地、 麦冬等组成。运用流感病毒A/PR/8/H1N1造成小鼠流感模型，以清营解表合剂干预感 染小鼠，运用整体观察、组织解剖和酶联免疫、流式细胞术等进行动物实验研究，通 过观察死亡保护率、肺组织病理、肺指数、肺组织病毒血凝滴度，验证其整体抗病毒 疗效；通过观察对淋巴细胞增殖功能、外周血细胞因子Th1/Th2变化的影响，探讨其 发挥作用的免疫机制。 结论：清营解表合剂对病毒感染小鼠有死亡保护作用，显著改善感染小鼠的肺组 织病变程度，降低肺指数、肺血凝滴度，证明整体疗效可靠。并能通过提高淋巴细胞 增值和Th1/Th2免疫调节提高流感病毒感染小鼠的免疫功能，从而对病毒感染小鼠起 保护作用。 关键词 感冒；养阴；实验研究；免疫调节；Th1/Th2 The Experimental Study of QingyingJiebiaoHeji s “Yangyin” Therapy on Coryza Speciality: Clinical foundation in Traditional Chinese Medicine Author: Sun XiWen Tutor: Lv CuixXia Abstract Purposes：This article will study the“Yangyin” therapy on Coryza through theoretical and experimental study to provide scientific evidence for the prevention and therapy from Coryza. Methods：This article presumes that external warm pathogen is the main etiological factor of External Hot Disease,through retrospecting the gnosia of pathogen and therapy about Coryza in TCM. It also poses the basical pathogenesis of Coryza:the external pathogen hurts lung first,and it turns up that body fluid is consumed in the initial stage,because it is easy for hot disease to consume body fluid firstly.According to such pathogenetic character,it establishes the treatment:Expelling the sufical symptoms using pungent and cool herbs firstly;Supplementing body fluid secondly.It sets up Qingyingjiebiaoheji which is composed of Honeysuckle flower, forsythia suspense, cynanchi atrati radix, rehmannia dride rhizome, ophiopogonis tuber etc.Finally,it studies its influence on the Th1/Th2 level in exterior blood. Result: QingyingJiebiaoHeji can protect the mouse infected by influenza virus and lower the