清肝益气降压方抗高血压血管Ⅰ、Ⅲ型胶原重塑的实验探究.pdfVIP

提 要 目的：通过观察清肝益气降压方对自发性高血压大鼠（SHR）尾动脉收缩压、动 脉血管胶原组织形态学改变、Ⅰ、Ⅲ型胶原及其调控因子转化生长因子β1 （TGF-β1） 的蛋白质及mRNA 表达的影响，探讨本方对高血压血管胶原重塑的干预机制，为原 发性高血压的治疗寻求有效治法和方药。方法：采用国际通用自发性高血压大鼠病理 模型灌胃给药，动态检测大鼠尾动脉收缩压，观察本方的降压作用；运用Masson 染 色法观察本方对胸主动脉血管胶原蛋白的形态改变；运用免疫组化技术和原位杂交技 术，观察本方对SHR 胸主动脉Ⅰ型胶原的蛋白质及其mRNA 表达，Ⅲ型胶原的蛋白 质及其mRNA 表达，胶原蛋白调控因子TGF-β1 蛋白质和mRNA 表达的影响。结果： 研究证明，本方有确切的降压作用，给药后与模型对照组相比血压明显下降（P ＜ 0.01 ）；与模型组相比，本方能够减少Ⅰ、Ⅲ型胶原的蛋白质及 mRNA 表达，降低 TGF-β1 蛋白质和mRNA 表达（P ＜0.01 ）。结论：清肝益气降压方具有良好的降压作 用，通过减少血管壁Ⅰ、Ⅲ型胶原的蛋白质及mRNA 表达来改善SHR 的血管胶原重 塑，其调控机制可能与降低TGF-β1 蛋白质和mRNA 表达有关。 关键词：清肝益气降压方；原发性高血压；血管胶原重塑；Ⅰ型胶原；Ⅲ型胶原 Experimental Research of Resisting Hypertensive Vascular Ⅰ and Ⅲ Collagen Remodeling by Antihypertensive Formula of Clearing Liver Fire and Supplementing Qi Speciality: Formulae of Traditional Chinese Medicine Author: Wang Yongrui Tutor: Professor Han Tao Abstract Objective: To observe the effect of antihypertensive formula of clearing liver fire and supplementing qi on systolic blood pressure, the histomorphology change of artery vascular collagen, the protein and mRNA expression of collagen Ⅰ、collagenⅢ and collagen modulators TGF-β1, the author wants to discuss the interferential mechanism on vascular collagen remodeling in SHR, to seek the effective therapeutic method and formula to treat primary hypertension. Methods: Adopting internationally SHR as pathological model, given drugs by inspire gastric. To observe the effect of antihypertensive by measuring the conscious SHR tail arterial systolic blood pressure by the tying tail method, Cutting of thoracis aorta of SHR into slices routinely, and dyeing the tissues of those with Masson method, the author observes the effect on the artery with t