第二届中国林业学术大会——S2 功能基因组时代的林木遗传与改良论文集C, 2009.pdfVIP

白桦核心种质初步构建 魏志刚 高玉池 刘桂丰 刘关君 杨传平 (东北林业大学林木遗传育种与生物技术教育部重点实验室 哈尔滨 150040) 摘 要：以白桦240 个家系的胸径、树高、材积和纤维素含量数据为依据，采用不同的抽样比率（5%、10%和 15%）、 2 种距离计算方法（马氏距离和欧氏距离）、8 种系统聚类方法(最短距离法、最长距离法、中间距离法、重心法、类 平均法、加权配对算术平均法、可变法和离差平方和法)和 3 种抽样方法(随机取样法、优先取样法和偏离度取样法) 构 建白桦初级核心种质资源，并利用均值差异百分率、方差差异百分率异、极差符合率和变异系数变化率评价不同方 法构建的核心种质。结果表明，采用 10%的抽样比例、马氏距离、最短距离系统聚类法和优先取样法构建的包括 24 个家系的白桦初级核心种质最能代表原有的种质群体。 关键词：白桦, 种质资源, 核心种质 Preliminary Construction Core Collection of Betula platyphylla Germplasm Wei Zhigang GaoYuchi Liu Guifeng Liu Guanjun Yang Chuanping (Key Laboratory of Forest Tree Genetic and Biotechnology of Ministry of Education, Northeast Forestry University Haerbin 150040) Abstract: Based on four quantitative traits which were height, diameter, volume and Cellulose content of 240 families of Betula platyphylla, the different primary core collections were constructed using different sampling proportion(5%、10% and 15%) , two distances (Mahalanobis distance and Euclidean distance), eight hierarchical cluster methods (Single linkage, Complete linkage, Median method, Centroid method, Unweighted pair-group method using arithmetic averages, Weighted pair-group average , flexible method and Ward’s method) and three sampling strategies (random sampling, preferred sampling and deviation sampling). The genetic variation of quantitative traits among these primary core collections was compared by evaluating the mean difference percentage( MD ), variance difference percentage ( VD ), coincidence rate of range (CR) and changeable rate of coefficient of variation ( VR ) of the traits. The results showed that the primary core collection composed of 24 families of Betula platyphylla and constructed w