食源性变形杆菌簇致病菌的检测-微生物所期刊联合编辑部.PDF

微生物学通报 JUN 20, 2010, 37(6): 839−844 Microbiology China © 2010 by Institute of Microbiology, CAS tongbao@ 研究报告 食源性变形杆菌簇致病菌的检测 * 陈双雅 张永祥 陈伟玲 王群力 谢明星 刘棠 彭小莉 (厦门出入境检验检疫局 福建 厦门 361026) 摘 要: 为建立食源性变形杆菌簇致病菌的检测方法, 对富集培养基、选择性平板分离培养基和 生化特性进行了筛选比较。结果表明, 肠杆菌富集肉汤培养基更有利于变形杆菌簇致病菌的检出, 平板分离培养基可用 EMB 琼脂培养基和 SS 琼脂培养基(或麦康凯琼脂培养基), 初筛方法为革兰 氏反应和苯丙氨酸脱氨酶实验, 最后用其他生化反应进行确认。该方法检出率高, 检测范围较宽, 可为建立变形杆菌簇致病菌的检验标准提供实验依据。 关键词: 变形杆菌簇, 致病菌, 检测 Detection of Food Borne Proteeae Pathogens * CHEN Shuang-Ya ZHANG Yong-Xiang CHEN Wei-Ling WANG Qun-Li XIE Ming-Xing LIU Tang PENG Xiao-Li (Xiamen Entry-Exit Inspect ion and Quarantine Bureau, Xiamen, Fujian 361026, China) Abstract: To establish a detection method of food borne Proteeae pathogens, we compared different enrichment broths and different selective agars on Proteeae pathogen growth, correlated with the bio- chemical characteristics of the pathogens. The results showed that EE enrichment broth was more effi- cient than GN broth, and the selective agars were EMB agar plus SS agar (or MacConkey agar). The preliminary screen methods were Gram-stain reaction and phenylalanine deaminase reaction. The Pro- teeae pathogens were finally confirmed by other biochemical tests. This method was sensitive and had a broad scope of detection, could provide experimental basis for new standard. Keywords: Proteeae, Pathogen