海洋鱼类血清游离皮质醇液质分析研究 - 宁波大学海洋学院.pdfVIP

36 ( FENXIHUAXUE) 7 2008 7 Ch inese Journal of Analytical Chem istry 05~ 0 1 2 * 1 1 2 1 1 徐继林 杨家锋 严小军 王亚军 尤仲杰 李海英 童玲 1 ( , 315211) 2 (, 315100) , , , V ( ) BV( ) = 1B3 --( UPLC-Q-TOF-M S) , m /z 407. 2 437. 2, BEH C , 0~ 200 L g/L 0. 3 18 1. 64 pg 3. 3% ~ 8. 1% , 10% (P reudosciaena Crocea) (Lateolabrax Japonicus) , 2. 11 ~ 75. 84 L g/L( Q-Q-Q-MS), ; 0~ 2000 L g/L, 0~ 200 L g/L , , , --, 1 , [ 1] , [ 2, 3] , , [ 4] , ; [ 1, 5] , , , --, , 2 2. 1 ACQU ITY , ACQU ITY ; Q-TOF Prem ier (WATERS); QuattroM icro( W aters); ( M ILLIPORE )( 8%, SIGMA-ALDRICH ); (, TED IA ); (, SIGMA-ALDR ICH ) 2. 2 2. 2. 1 5. 0 mg, 1. 0 mL, , 5 g/L , 4 e V() BV() = 1B1 , 50 Lg /L 2. 2. 2 (Preudosciaena Crocea) (Lateolabrax Japonicus): 2007 4 , , 0. 5 mL, 12000 r/m in 2007-10-12; 2008-01-16 ( 12005 2262-0505) ( 705028) * E-m ail: yanxiaojun@ nbu. edu. cn 36 06 , 5 LL 5 mg /L , , 0. 5 mL 0. 25 mL, 5 m in, 12000 r/m in, , , , 0. 1 mol/L N aOH , 2 , , V() BV (H O) = 1B3 2 0. 50 mL, , 2. 2. 3 UPLC: ACQU ITY UPLC BEH C ( 100 mm @ 2. 1mm, 1. 7 Lm) (