正交试验法优化微波协同双水相提取甘草中总黄酮的工艺 - 中国药房.pdf

正交试验法优化微波协同双水相提取甘草中总黄酮的工艺 1＊ 2# 2 陈丽娟 ，柳福智 ，岳强兵 （1.甘肃奇正藏药有限公司，兰州 730100；2.甘肃农业大学农学院，兰州 730070） 中图分类号 R284.2 文献标志码 A 文章编号 1001-0408（2016）04-0525-04 DOI 10.6039/j.issn. 1001-0408.2016.04.29 摘 要 目的：优化微波协同双水相提取甘草中总黄酮的工艺。方法：以硫酸铵和乙醇为双水相萃取体系，微波法提取甘草中的 总黄酮；采用单因素试验和正交试验考察硫酸铵质量浓度、乙醇体积分数、固液比、微波功率、提取时间5个因素对总黄酮提取率 的影响，优化提取工艺并进行验证试验。结果：最优提取工艺为硫酸铵质量浓度0.40g/ml、乙醇体积分数70％、固液比1∶25、提取 时间3min 、微波功率300W ；验证试验中甘草总黄酮平均提取率为2.038％（RSD ＝0.69％，n ＝3），双水相萃取率为94.46％ （RSD ＝1.30％，n ＝3）。结论：该工艺操作简单、结果稳定、反应条件温和、提取效率较高，适合工业化提取甘草中总黄酮。 关键词 正交试验；微波；双水相；甘草；总黄酮 Technology Optimization of Microwave-assisted Aqueous Two-phase System of Total Flavonoids from Glyc- yrrhiza uralensis by Orthogonal Test 1 2 2 CHEN Lijuan ，LIU Fuzhi ，YUE Qiangbing （1.Limited Company of Gansu Chee Zheng Tibetan Medicine ，Lan- zhou 730100，China ；2.College of Agriculture ，Gansu Agricultural University ，Lanzhou 730070，China ） ABSTRACT OBJECTIVE ：To optimize microwave-assisted aqueous two-phase system of total flavonoids from Glycyrrhiza ura- lensis. METHODS ：The ammonium sulfate and ethanol were used as aqueous two-phase extraction system ，the method of micro- wave was applied to extract total flavonoids from G. uralensis. The effects of 5factors on extraction rate of total flavonoids were in- vestigated by single factor test and orthogonal test ，such as the mass concentration of ammonium sulfate ，ethanol volume fraction ， solid-liquid ratio ，microwave power and extraction time. Validation test was conducted. RESULTS ：The optimal extraction condi- tion was as follows as the mass concentration of ammonium sulfate of 0.40 g/ml ，ethanol volume fraction of 70％，solid-liquid ra- tio of 1∶25，extraction time of 3 min and microwave power of 300 W ；in validation test ，the extraction rate of total flavon