作物数量性状基因(qtl)克隆研究进展 - 中国农学通报.pdfVIP

中国农学通报 2011,27(7):210-215 Chinese Agricultural Science Bulletin 作物数量性状基因(QTL)克隆研究进展 1,2 3 2 1 周 雷 ，胡广隆 ，李自超 ，游艾青 1 2 （湖北省农业科学院粮食作物研究所，武汉430064 ；中国农业大学植物遗传育种系，北京100193； 3 北京市农林科学院林业果树研究所，北京100093） 摘 要：此文首先简述了数量性状基因（QTL ）克隆研究方法，然后较全面的总结了作物QTL 克隆的研究 进展，最后讨论了QTL 调控的分子机理。对37 个已成功克隆的QTL 进行汇总分析表明：控制作物数量 性状的有转录因子、蛋白激酶、细胞分裂素氧化酶类等基因；其中12 个已成功克隆的QTL 编码转录因 子，说明转录调控因子是新的植株形态进化和变异的重要原因。随着测序技术的发展，海量的基因组数 据将为QTL 的遗传定位和分子剖析提供更多便利，越来越多的QTL 将会被克隆出来。 关键词：作物；QTL ；基因克隆；转录因子 中图分类号：S-1，Q-1 文献标志码：A 论文编号：2010-2555 AdvanceinCropQTLCloning 1,2 3 2 1 Zhou Lei , Hu Guanglong , Li Zichao , You Aiqing 1 ( FoodCropsInstitute,HubeiAcademyofAgriculturalSciences, Wuhan 430064; 2DepartmentofPlantGeneticsandBreeding,ChinaAgriculturalUniversity, Beijing 100193; 3 InstituteofForestryandPomology,BeijingAcademyofAgricultureandForestrySciences, Beijing 100093) Abstract: The strategy, progress and molecular mechanism of crop QTL cloning were reviewed in this paper. Summarized 37 successfully cloned QTLs in crops, we found that the quantitative traits were controlled by a few kinds of genes, such as transcription factors, protein kinase, cytokinin oxidase, etc. More over, 12 of the 37 genes were transcription factors, which suggested that transcription factors are important reasons for plant evolution and variation. With the development of sequence techniques, much more advantage for QTL mapping