electrospray ionization for mass spectrometry of large biomolecules（电喷雾电离质谱的大生物分子）.pdf

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electrospray ionization for mass spectrometry of large biomolecules（电喷雾电离质谱的大生物分子）.pdf

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electrospray ionization for mass spectrometry of large biomolecules（电喷雾电离质谱的大生物分子）

Journal of Biomolecular Techniques 13:101–118 © 2002 ABRF A B RF REVIEWS systems. Those leaks had turned out to be very pow- Electrospray Ionization Mass erful and versatile research tools because they could produce molecular beams with much higher energies Spectrometry: How It All Began* and intensities than could the effusive sources with which Otto Stern and his disciples had earned half a John B. Fenn dozen Nobel prizes in physics. Moreover, the super- sonic free jets issuing from these leaks introduced their component gases to nonequilibrium steady Chemistry Department, Virginia Commonwealth states having combinations of temperature and den- University, 1001 West Main St., Richmond, VA sity not previously dreamt of, let alone encountered. Those supersonic free jets subsequently ushered in the era of electrospray ionization (ESI) in mass spec- tromet