cdna文库（CDNA library）.docVIP

cdna文库（CDNA library） Using mRNA as template, the reverse transcriptase catalyzed in vitro reverse transcription into cDNA with the appropriate carrier (commonly used phage or plasmid) was transformed into connection, each bacterium contains a cDNA, and can reproduce this amplification, contains all the mRNA information of the cDNA cell clone set is called a cDNA library the tissue cells. The genome contains genes in specific tissues only a part of expression, and in the types and intensity of expression of different environmental conditions and different differentiation period the genes are not the same, so the cDNA library has tissue specificity. The cDNA library is obviously much smaller than the genomic DNA library and can be easily screened for specific cell expression genes. But in eukaryotic cells, obtained from genomic DNA library and cDNA Library of different gene obtained from the genomic DNA library contains the intron and exon with genomic gene, obtained from the cDNA library that has been removed and splicing of intron cDNA. The eukaryotic genome DNA is very large, and its complexity is about 100 times of mRNA and protein, but also contains a large number of repeat sequences. By using the method of electrophoresis and hybridization, are difficult to directly isolated gene. This is one of the main difficulties from chromosome DNA as the starting materials for direct cloning gene. Higher organisms in general with about 105 different genes, but a single cell or individual in a certain time period, all 15% genes to be expressed, produces about 15000 different mRNA molecules. Therefore, cloning of cDNA mRNA starting from the complex than directly from the genomic clone much simpler. Edit this paragraph definition (cDNA Library) mRNA a biological genome transcription by reverse transcription cDNA fragments generated respectively with recombinant cloning vector, and then introduced into the corresponding host cell (E.coli.) propagation and amplification theory,