太阴病脾阳虚证的唾液菌群特征研究.pdfVIP

2008年第26卷第6期 四 川 中 医 Vo1．26，No．6，2008 Journal of Sichuan of Traditional Chinese Medicine · 7 · 太阴病脾阳虚证的唾液菌群特征研究 姜冬云 王 笳2 1．成都中医药大学 (四川 成都610075) 2．四川省中医药研究院 (四川成都610041) 摘要：目的：探讨脾阳虚患者和正常人口腔唾液茵群细茵种类及数量的差异，进而揭示脾阳虚证唾液茵群种类、 数量、分布及特征性变化规律。方法：参照 《中药新药临床研究指导原则》的临床实施标准，将确诊的脾阳虚证患 者，纳入本实验。采用需氧、厌氧和真茵培养方法对脾阳虚患者口腔唾液样本进行定性和定量检测。结果：除真茵 外，口腔各正常细菌的检出率 (％)和CFU／ml较正常人垮有不同程度的下降。其中，葡萄球菌、产黑色素的革兰阴 性的无芽胞厌氧杆茵、奈瑟氏茵三种细茵在捡出率 (％)和CFU／ml上与正常人均有显著差别 (P0．05)；唾液链球 茵的检出率 (％)和CFU／ml在脾阳虚组与正常组无明显差异 (P0．05)。结论：葡萄球茵、产黑色素的革兰阴性的 无芽胞厌氧杆茵、奈瑟氏茵检出率％和CFU明显下降 (P0．05)是太阴病脾阳虚证口腔真茵群的基本特征之一。 关键词：太阴病 脾阳虚 口腔茵群 微生物学 中图分类号：R241 文献标识码：A 文章编号：1000—3649(2008)06—0007—02 Study on the Saliva Flora of Patients with Hyposplenoyang Syndrome／Jiang Dongyun Wang Jia ∥1．Chengdu University of Tra· ditional Chinese Medicine (Chengdu Shuan 610075，China)2．Sichuan Academy of Chinese Medwine Sciences(Chengdu Sichuan 610041。China) Abstract：Objective：To discuss the differences in the categories and magnitude of bacteria in saliva flora between Hypos— plenoyang patients and healthy persons，and then to reveal the categories，magnitude，distributing and diversification laws of saliva flora of Hyposplenoyang syndrome．Methods：Hyposplenoyang patients were diagnosed according to the standard in Clinical Guiding Princi— ples for TCM New Drugs．The saliva flora of Hyposplenoyang patients and healthy persons were determined qualitatively and quantita· tively in the methods of aerobic。anaerobic and fungicidal incubation．Results：The detection ratio(％)and CFU／ml of normal bacte· ria except epiphyte in the mouths of Hyposplenayang patients were ower with different extent than that of healthy persons The detection ratio(％)and CFU／ml of staphylococcus。melanin—producing Gn non—sporangium anaerobic bacilus and