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Supplemental Figure 1
Figure S1. Alignment of the MYB (A) and predicted coiled–coil (B) conserved
domains constructed by use of the CLUSTAL X 1.81 program (Thompson et al., 1997)
and colored by use of the GeneDoc 3.2 program with default BLOSUM score.
Supplemental Figure 2
Figure S2. Growth performance of 45-d-old wild type (WT) and one line of
OsPHR2-overexpressing plants (OsPHR2-Ov-1) in a pot experiment using acidic red
soil supplied with 3 Pi levels: A, 30 mg Pi/Kg soil; B, 60 mg/Kg soil; C, 120 mg
Pi/Kg soil; D, 200 mg Pi/Kg soil.
Supplemental Figure 3
Figure S3 Growth parameters measured from wild type (white column) and
OsPHR2-Ov-1 (black column) plants in the soil pot experiment with four Pi levels. A,
Plant height; B, Panicle length; C, Maximum tiller number; D, Seed-setting tiller
number; E, Seed-setting rate; F, Grain number; G, Chlorophyll. Values are mean ±SD
(n=6). Stars on the bars represent means that are statically different between WT and
OsPHR2-Ov-1 in the same treatment (p0.01).
Supplemental Figure 4
Figure 4. A, Root hair proliferation of OsPHR1-RNAi and OsPHR2-RNAi lines in
the regions of root base (left panels), elongation zoon (middle panels) and primary
root tip (right panels) grown under Pi-supplied (b,2Ri-1 and c, 1Ri-1) and
Pi-deficient (e,2Ri-1 and f, 1Ri-1) conditions compared with WT (a and d). B UP, Pi
concentration in the shoots and roots of wild type, 2Ri-1 and 1Ri-1; Down, Pi
concentration in the shoots and roots of wild type, 2Ri-1 and 1Ri-1. Error bars
indicate the SD (n=5). C Left, 2Ri-1 and 1Ri-1 after grow under high Pi conditions
(10mg/L Pi, 0.5L/plant) for 30 days; Right, 2Ri-1 and 1Ri-1 after grow under low Pi
conditions (1mg/L Pi, 0.5L/plan
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