更年春方对去卵巢大鼠胶原纤维作用的研究.pdfVIP

4 更年春方对去卵巢大鼠胶原纤维作用的研究 袁怡清 王文君 周文江 李大金 (复旦大学附属妇产科医院，上海 200011) [摘 要] 目的 观察更年春方(GNC)对去卵巢大鼠皮肤组织胶原纤维的作用并探讨可能发生的机制。方法 1O～12月龄sD雌性大鼠6o 只随机分为假手术组(s组)、生理盐水组(N组)、GNC高剂量组(H组)、GNC低剂量组(L组)、尼尔雌醇组(E组)，每组12只。除s组做假性手术 外，其余各组均行双侧卵巢切除术。术后2 w开始给予灌药，N组灌服生理盐水，H组灌服GNC 2 ml／d、L组灌服GNC 1 ms／d、E组灌服尼尔雌醇 1 ml，1次／w。3个月后测定大鼠血清雌二醇(E2)的水平，取大鼠背部皮肤组织进行组织学及免疫组织化学观察，测定皮肤组织羟脯氨酸(HYP)水 平，RealTime—PCR定量分析皮肤组织表皮细胞生长因子受体(EGFR)、ER亚型mRNA的表达。结果 GNC及尼尔雌醇均能提高大鼠皮肤组织HYP 含量，提高皮肤组织EGFR、ERct、ER~mRNA的表达，血清E2水平均未升高。结论 更年春方能增加去卵巢大鼠皮肤组织胶原纤维的表达，此作用 与GNC影响胶原蛋白合成途径有关。其机制可能与提高皮肤组织ERmRNA的表达有关。 [关键词] 更年春方；皮肤；胶原；表皮生长因子受体(EGFR)；雌激素受体亚型 [中图分类号] R965 [文献标识码] A [文章编号]1005-9202(2008)04-0313-04 ‘ Effects of Gengnianchun formula on skin collagen fibers in ovariectomized SD rats YUAN Yi-Qing，WANG Wen-Jun，ZHOU Wen-Jiang，et以 Obstetric and Gynecology Hospital，Fudan University，Shanghm 200011，China 【Abstract】 Objective To study the possible effects of Gengnianehun formula(GNC)on skin collagen in ovariectomized(ovx)SD rats and to investigate its possible mechanism．Methods 60 ten to twelve months old SD rats were randomly divided into sham—operated (S)，saline(N)，hish(H)and low(L)dosage of GNC and nilestriol(E)groups，Except S group，the rats in the other groups ovariecto— mized bilaterally 2 weeks ago received corresponding drugs，respectively．Serum E2 levels were determined 3 months later in five groups．Skin tissue of the back area were got for histological and immunohistological study and measurement of hydmxypmline(HYP)．Results PCR was performed to analyze the expression of epidermal growth factor receptor(EGFR)，E胁，ER[3 of skin tissue．Results I1Ile amount of HYP sig— nifieanfly increased after treated with GNC or nilestrio1． I1Ile mRNA level of EGFR，ER0【，ER[3 of skin tissue all enhanced dramatiely after treated with GNC or nilestrio1．rI1Ile serum E2 level had no significant increase．Condusions GNC could improve the am ount of skin co