细胞凋亡的几种检测方法2（Several methods of detecting apoptosis 2）.docVIP

细胞凋亡的几种检测方法2（Several methods of detecting apoptosis 2） Several methods of detecting apoptosis 2.txt32 because love, wandering people can return to their homes; because love, tired soul, vitality. Hunger, love, like the stars, desire to shine, love, like the song of the century, to be sung for ever. Several methods of detecting apoptosis Morphological observation method 1, HE staining and light microscope observation: the apoptotic cells were round, the nuclei were stained deeply, the cytoplasm was condensed, the chromatin became clumps, and the cell surface had budding phenomenon. 2 、 acridine orange (AO) staining, fluorescence microscopy showed that the living cells were yellow green fluorescent, and the cytoplasm was red fluorescent. The chromatin of apoptotic nuclei was yellow green and concentrated in the inner membrane of the nuclear membrane. The cell membrane showed vesicular bulge and apoptotic bodies. 3 trypan blue staining: if the cell membrane is incomplete and broken, trypan blue dye enters the cell and the cell becomes blue, which is necrosis. If the cell membrane is intact and the cells are not trypan blue, they are normal or apoptotic cells. This method is helpful to reflect the integrity of the cell membrane and differentiate the necrotic cells. 4 observation of transmission electron microscope: the apoptotic cell surface microvilli disappeared, chromatin condensation and margination, usually crescent shaped, membrane folds, cytoplasmic organelles, compaction, cell membrane blebbing or corpuscles shoots and apoptotic bodies and apoptotic macrophages were near phenomenon. Two and DNA gel electrophoresis (1) detection principle; Apoptosis or necrosis occurred in the cells, and all of the cells of DNA broke down, and the intracellular small molecular weight DNA fragments increased, DNA decreased, and DNA fragments appeared in cytoplasm. But the apoptosis DNA were fracture occurred in the regular nucleosome, appear 180200bpDNA fragment, and DNA cells wit