式细胞术在淋巴增殖性疾病中的应用.pptVIP

流式细胞术在淋巴增殖性疾病中的应用;WHO2008淋巴增殖性疾病分类(1);WHO2008淋巴增殖性疾病分类(2);WHO2008淋巴增殖性疾病分类(3);2006 Bethesda 会议共识（1）;2006 Bethesda 会议共识（2）;2006 Bethesda 会议共识（3）;FCM studies for mature B neoplasms;FCM studies for mature B neoplasms;FCM studies for mature B neoplasms;FCM for mature T/NK neoplasms;淋巴瘤/淋巴增殖性疾病重要抗原;Mature lymphoid neoplasias;Tissue based lymphoma;Circulating LPD;FCM在淋巴瘤/LPD诊断中的地位;Identification abn T/NK cells（1）;;Identification abn T/NK cells（2）;;CD5/CD19/CD45;;Identification abn T/NK cells（3）;;Progression in some distinct disease entities. Mary remain in PTCL, U Diagnosis depend multiple diverse pieces of information. Morphologic review IHC FCM Cytogenetic sudies Molecular studies. Clinical information ;Role of FCM in the classification of mature T cell neoplasms（2）;Morphologic assessment is essential AITCL Growth around residual LN ALCL Anaplastic morphologic appearance LGL Large granular lymphocyte;Immunophenotype are important parts FCM and/or IHC Select the optimal techniques: Are the neoplastic cells T or NK cell FCM better than IHC： Flow CD3 for T ,not for NK IHC CD3ε can not distinguish T and NK Is there expression CD4 or CD8 FCM better than IHC ;Select the optimal techniques: NK associated Ag. CD16, CD56, CD57 FCM more sensitive CD16 is not currently available in IHC Components of the TCR FCM are the optimal method for determining αβ/γδ Γδ TCR could not available in IHC Cytotoxic granule: TIA-1, granz-B，porforin Either by FCM or IHC Phenotypic feature with ALCL CD30 and Alk-1 : IHC　better ;Select the optimal techniques: Is the lymphoma EBV associated? IHC and paraffin section better Whether express CD103? EATCL CD103+ CD103 can’t detected by IHC ;Classification Mature T cell neoplasms;CD4+CD8-neoplasms（1）;CD4+CD8-neoplasms（2）;CD4+CD8-neoplasms（3）;66 m，肩、胸部持续皮疹。PB：WBC15X109/L, 60% LC, Hb、plt 正常。;;CD4+CD8-neoplasms（5）;77,male, presented with splnomegaly, lymphadnopathy, and marked absolute lymphocytosis. ;朗冕泽志巫却应抉缕严渠氢胯肖盅形名档罩亨述边忆奸彭螺灿磺鸯话出靖式细胞术在淋巴增殖性疾病中的应用式细胞术在