增强uv鄄b 辐射对小麦根尖分裂期细胞肌动蛋白的影响 - plant diversity.pdfVIP

植 物 分 类 与 资 源 学 报摇 2011,33 (3):306~310 Plant Diversity and Resources摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 DOI:10.3724/ SP.J.1143.2011.10180 增强UV鄄B辐射对小麦根尖分裂期细胞肌动蛋白的影响* 陈慧泽, 翟菁如, 杜美婷, 韩摇 榕** (山西师范大学生命科学学院, 山西 临汾摇 041004) -2 -1 摘要: 以增强UV鄄B (10.08kJ·m ·d ) 辐射后的小麦根尖细胞为材料, 异硫氰酸荧光素标记的鬼笔环肽 (FITC鄄Ph) 为探针, 利用激光共聚焦扫描显微镜, 观察分析小麦根尖分裂期细胞肌动蛋白在分裂周期的 分布及形态变化。 结果表明: UV鄄B辐射处理后, 间期细胞肌动蛋白纤丝排列紊乱; 前期细胞周质中肌动 蛋白纤丝变为短的片段, 点状荧光随机分布在周质; 中期细胞荧光强度明显减弱, 明亮的肌动蛋白片段消 失, 点状荧光消失; 有丝分裂后期到末期, 成膜体区域肌动蛋白纤丝或成弥散状或完全消失, 且出现落后 染色体、 染色体桥、 不均等分裂及三束分裂等染色体畸变类型和异常分裂现象。 关键词: 增强UV鄄B; 小麦; 分束分裂细胞; 肌动蛋白; 激光共聚焦扫描显微镜 中图分类号: Q942摇 摇 摇 摇 摇 摇 文献标识码:A摇 摇 摇 摇 摇 摇 摇 文章编号:2095-0845(2011)03-306-05 Influence of Enhanced UV鄄B Radiation on F鄄actin in Wheat Division Cells CHEN Hui鄄Ze,ZHAI Jing鄄Ru,DU Mei鄄Ting,HAN Rong** (College of Life Science,ShanxiNormal University,Linfen041004,China) Abstract:The distribution of F鄄actin was examined by confocal laser scanning microscopy (CLSM) in the wheat -2 -1 root鄄tip cellsundertheenhancedultraviolet鄄B (10.08kJ·m ·d )radiationtreatedwith FITC鄄Phalloidin. There鄄 sults showedthatundertheconditionoftheenhancedultraviolet鄄Bradiation,distributionof actinMFsweredisorder鄄 edininterphasecells;ThearraysoftheF鄄actindisintegratedintoshortfragmentsofrandomduringprophase;Fluores鄄 cence intensity significantly decreased and bright fragments of microfilaments disappeared in Metaphase cells;Actin MFs disappearedinthephragmoplastregionswhencellswereinanaphaseandtelophasetime. Andsometypesofchro鄄 mosome aberrationwere induced,including Chromosomal logging,chromosomal bridge and partition鄄bundle division. Key words:Enhanced UV鄄Bradiation;Wheat;Partitionbundle divisio