龙须菜藻红蛋白对hela 细胞增殖抑制及其机制的研究 - 食品科学.pdfVIP

※营养卫生 食品科学 2007, Vol. 28, No. 0 549 陈美珍，葛安山，崔鹏举，廖灶辉 (汕头大学理学院生物系，广东 汕头 515063) ：目的：探讨龙须菜藻红蛋白(PE)对人宫颈癌细胞Hela 体外的抑制作用及其作用机制。方法：采用MTT 法检测不同浓度的藻红蛋白对Hela细胞增殖抑制效果。流式细胞仪和Annexin V-FITC / PI双荧光染色法观察藻红蛋 白对Hela细胞周期及细胞凋亡的影响。结果：PE可显著抑制 Hela细胞的生长，并呈剂量－效应关系 (p＜ 0.01， r=0.84)，剂量为20μg/ml，作用48h，其抑制率达70.88%，其48h的IC50 值为4.12μg/ml。PE可阻滞Hela细胞从 G2/M 期进入S 期，诱导细胞凋亡。结论：PE 对 Hela 细胞有较强的抑制作用，其作用机制部分与诱导 Hela 细胞调 亡有关。 ：龙须菜藻红蛋白； Hela 细胞；细胞调亡 Study on Inhibition Effects of Phycoerythrin from Gracilaria lemaneiformis on Hela Cells and Its Mechanism CHEN Mei-zhen，GE An-shan，CUI Peng-ju，LIAO Zao-hui (Department of Biology, College of Science, Shantou University, Shantou 515063, China) Gracilaria lemaneiftheormis on growth Abstract ：Objective: To investigate the inhibition effects of phycoerythrin (PE) from of Hela cells and its mechanism. Method: The proliferation of cell lines of Hela in vitro was assayed by MTT method. Effects of PE on Hela cells cycle and apoptosis were observed by flow cytometry and Annexin V-FITC/PI fluorescence staining. Result: PE could obviously inhibit the proliferation of Hela cells with a fair dose-effect relationship (p＜0.01，r =0.84). The inhibitory rate at 48 h could reach more than 70% and its half inhibitory concentration (IC50) was 4.12μg/ml. PE could prevent the transformation of Hela cell cycle from G2/M to S phase to cause apoptosis. Conclusion: PE shows potent inhibitory effects on growth of Hela cells, and the mechanism is related to inducing the apoptosis of Hela cells. Key words：phycoerythrin from Gracilaria lemaneiformis；Hela cell；apoptos