滨梅茎段组织培养研究.PDFVIP

安徽农业大学学报, 2012, 39(5): 816-820 Journal of Anhui Agricultural University 网络出版时间：2012-8-27 11:55:40 [URL] /kcms/detail/34.1162.S1155.004.html 滨梅茎段组织培养研究 胡淑英，王小敏，张春红，吴文龙，李维林，汤飞云 (江苏省中国科学院植物研究所，南京 210014) 摘 要：以滨梅（Prunus maritima Marshall ）带腋芽茎段为外植体进行了组织培养技术的研究。结果表明，植 物生长调节物质种类及浓度、碳源、光照度对滨梅不定芽增殖和生长均有显著影响。综合分析单因素试验和正交试 验结果表明，在滨梅组织培养中，最佳初代培养基为 MS+0.5 mg·L-1 TDZ ；最佳继代增殖培养基为 MS+0.3 mg·L-1 TDZ +0.1 mg·L-1 -1 -1 2,4-D ；最佳生根培养基为 1/2 MS + 0.2 mg·L NAA 。培养基以质量浓度为 30 mg·L 的葡萄糖为碳 源，光照度为 3 000 lx。 关键词：滨梅；组织培养；植物生长调节物质；碳源；光照度 中图分类号：Q945.52 文献标识码：A 文章编号：1672−352X (2012)05−0816−05 Tissue culture with the stem of Prunus maritima Marshall HU Shu-ying, WANG Xiao-min, ZHANG Chun-hong, WU Wen-long, LI Wei-lin, TANG Fei-yun (Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014) Abstract: The research was conducted on in vitro culture of Prunus maritima Marshall with stem segments as explants. The results showed that the kinds and concentrations of plant growth regulator, carbon source, and illumination intensity have significant effects on shoot proliferation and growth. Based on single factor and or- -1 thogonal tests, we concluded that the optimum medium for explant growing is MS+0.5 mg·L TDZ; the optimum -1 -1 medium for proliferation is MS+0.3 mg·L TDZ +0.1 mg·L 2, 4-D; the optimum medium for rooting is 1/2 MS + -1 -1 0.2 mg·L NAA. In every cultivation stage, it is more suita