蛋白分型：一种在蛋白水平研究流感病毒进化的新方法.pdfVIP

VIROLOGICA SINICA, October 2007, 22 (5):405-411 CLC number: Q78 Document code: A Article ID: 1674-0769(2007)05-0405-07 Proteotyping: A New Approach Studying Influenza Virus Evolution * at the Protein Level 1 2 3 4 5 Wei-feng SHI , Zhong ZHANG , Lei PENG , Yan-zhou ZHANG , Bin LIU and Chao-dong ZHU4** (1. Institute of Life Sciences, Taishan Medical College, Shandong Tai’an, 271000 China; 2. Department of Basic Medicine, Taishan Medical College, Shandong Tai’an, 271000 China; 3. College of Information and Engineering, Taishan Medical College, Shandong Tai’an, 271000 China; 4.Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101 China; 5. Department of Biological Sciences, Taishan Medical College, Shandong Tai’an, 271000 China) Abstract: Phylogenetic methods have been widely used to detect the evolution of influenza viruses. However, previous phylogenetic studies of influenza viruses do not make full use of the genetic information at the protein level and therefore cannot distinguish the subtle differences among viral genes. Proteotyping is a new approach to study influenza virus evolution. It aimed at mining the potential genetic information of the viral gene at the protein level by visualizing unique amino acid signatures (proteotypes). Neuraminidase gene fragments of some H5N1 avian influenza viruses were used as an example to illustrate how the proteotyping method worked. Bayesian analysis confirmed th