真菌子实体形成需要.pptVIP

An eight-subunit COP 9 signalosome with an intact JAMM motif is required for fungal fruit body formation;An eight-subunit COP 9 signalosome with an intact JAMM motif is required for fungal fruit body formation;子实体(fruitingbody)：当营养生活进行到一定时期时，真菌就开始转入繁殖阶段，形成的各种繁殖体。 光形态建成：由光控制植物生长,发育和分化的过程。 COP9：一个光调控植物发育的分子开关。 信号传导体：一类通过与细胞受体结合发挥作用，将信息传导到细胞核中，激活特定基因的物质。 COP9 signalosome：简称为CSN JAMM蛋白基序：来源于JAB1/MPN/MOV34 metalloenzyme，是CsnE上的结构域。;Fruit body formation in filamentous fungi（丝状真菌）is a complex and yet hardly understood process. We show here that protein turn over （周转）control is crucial for Aspergillus nidulans（钩巢曲霉） development. Deletion of genes encoding COP9 signalosome (CSN) subunits 1, 2, 4, or 5 resulted in identical blocks in fruit body formation. The CSN multiprotein complex（多蛋白复合体）controls ubiquitin-dependent protein degradation （泛肽依赖性蛋白降解）in eukaryotes. ;Six CSN subunits interacted in a yeast two-hybrid analysis（酵母双杂交分析）, and the complete eight-subunit CSN was recruited （使恢复）by a functional tandem affinity purification（串联亲和纯化）tag（标记） fusion （融合）of subunit 5 (CsnE). The tagged CsnE was unable to recruit any CSN subunit in a strain deleted for subunit 1 or subunit 4. ;Mutations （突变）in the JAMM metalloprotease （金属蛋白酶）core of CsnE resulted in mutant phenotypes （显型） identical to those of csn deletion strains（缺失突变株系）. CsnE中心的JAMM金属蛋白酶发生突变导致与csn缺失突变株系同样的突变显型。（ csn表示基因） We propose that a correctly assembled （装配） CSN including a functional JAMM links protein turnover to fungal sexual development. 我们认为包括一个功能JAMM的CSN的正确装配联系到真菌有性繁殖的蛋白周转。;Fungal fruit bodies are sexual reproduction structures that generate meiotic spores（减数分裂的孢子）. The model mold Aspergillus nidulans （钩巢曲霉） develops a closed spherical fruit body （封闭的球状子实体） (cleistothecium（闭囊壳） ) including different tissue types: Hülle cells surround and nurse the growing cleistothecium, pericarp cells develop the protecting wall, and inner ascogenous（产囊）cells mature into sexual spores. Massive reconstruction of vegetati