1复制与转录过程有什么相似之处.pptVIP

RNA Biosynthesis (Transcription);1．复制和转录过程有什么相似之处？又各有什么特点？2. 试述真核细胞RNA转录后的加工修饰 ; Concept The process of copying DNA to RNA by an enzyme called RNA polymerase (RNAP) The transfer of genetic information from DNA into RNA;Content;The difference between replication and transcription;Substances for transcription; Templates and Enzymes;Template for transcription;5′···GCAGTACATGTC ···3′;5? 3?;Asymmetric transcription ;RNA polymerase;core enzyme;The binding site of RNApol holoenzyme on the transcription initiation region;Eukaryotic RNA polymerase;A site for RNA pol to identify and bind on the template ;RNA polymerase protectionassay;Transcription initiation; TATA bax; The Process of Transcription;Initiation; The DNA is unwound and becomes single-stranded in the vicinity of the initiation site (defined as +1). ;Elongation;transcription bubble：;;5?;Rho (ρ) factor dependent transcription termination Rho-independent transcription termination;A T P;Termination independent on Rho factor ;5`UUGCAGCCUGACAAAUCAGGCUGAUGGCUGGUGACUUUUUAGUCACCAGCCUUUUU... 3` ;Mechanism involved in the termination by stem loop;Eukaryotic transcription initiation;Transcription Initiation n site;Transcription factor;参与RNA-polⅡ转录的TFⅡ ;Pre-initiation complex ( PIC);POL-Ⅱ;Piecing theory ;Elongation;RNA-Pol;5?------AAUAAA-; Post-transcriptional modification;Concept In eukaryotic cells, a genetic process for the conversion of a primary transcript RNA to mature RNA;Major modifications;Eukaryotic mRNA processing;5? cap structure;5? pppGp…;mRNA splicing;a strand of DNA containing both introns and exons. Most advanced eukaryotes have interrupted genes and introns are longer than exons. The resulting gene is much longer than its coding region;Exon and intron;Chicken Ovalbumin Gene ;Electron-microscopic picture of the mature mRNA and DNA hybridization of Chicken Ovalbumin ;Classification of introns ;mRNA splicing;②;pG-OH (ppG-OH, pppG-OH);Differential RNA processing: RNA edi