的拮抗作用.PDF

·论 著 · 血管紧张素Ⅱ对大鼠肾入球小动脉细胞内钙离子浓度的影响及 Losartan 的拮抗作用 孙洞箫，周盾，邱振宇(辽宁医学院附属第一医院肾内科，辽宁 锦州 121001) 摘要：目的 探讨血管紧张素 Ⅱ(Ang Ⅱ)对肾小球入球小动脉细胞内钙离子浓度([Ca2+]i)的影响与高血压肾小动脉重建的 关系及 Losartan 的治疗作用。方法 细胞培养：大鼠肾小球入球小动脉细胞随机分为 4 组。对照组：不加 Ang Ⅱ处理； Ang Ⅱ组：加入终浓度为 0.1 μmol·L1 的 Ang Ⅱ；Losartan 组：加入终浓度为 50 μmol·L1 的 Losartan ；Ang Ⅱ+Losartan 组： 同时加入 Losartan 50 μmol·L1 和 Ang Ⅱ0.1 μmol·L1。负载后上机检测[Ca2+]i 。结果 细胞培养显示 Ang Ⅱ引起了细胞收 缩变化，表现为胞突变细变短，胞突回缩，胞体变圆，细胞长度与直径均明显缩小。Losartan 处理组较 Ang Ⅱ组细胞形 态变化减轻且 Losartan 对 Ang Ⅱ增高 RASMCs 内[Ca2+]i 有明显抑制作用。结论 Ang Ⅱ可引起肾小球入球小动脉细胞内 [Ca2+]i 的上升，导致细胞收缩，因此 Ca2+超载可能是肾素-血管紧张素系统起作用的重要环节。Losartan 可抑制细胞内 Ca2+ 超载。 关键词：肾小球入球小动脉细胞；高血压；血管紧张素Ⅱ；Losartan ；细胞内钙离子浓度 中图分类号：R962.1 文献标志码：A 文章编号：1007-7693(2010)10-0857-04 Effect of Angiotensin II on Intracellular Free Calcium Concentration in RASMCs and Antagonism of Losartan SUN Dongxiao, ZHOU Dun, QIU Zhenyu(Department of Nephorology, the First Hospital Affiliated Liaoning Medical College, Jinzhou 121001, China) ABSTRACT: OBJECTIVE To explore the effects of angiotensin(Ang Ⅱ ) on intracellular free calcium concentration([Ca2+]i) in RASMCs and antagonism of Losartan. METHODS RASMCs were isolated and divided into four groups: control group, Ang Ⅱ group, Losartan group and Ang Ⅱ +Losartan group. Confocal microscope was used with Fluo-3/AM as an indicator to detect the changes of [Ca2+]i. RESULTS Compared with control group, intracellular fluorescent intensity of RASMCs had no change in Losartan group, and was significantly higher in Ang Ⅱ group (P0.05). Value in Ang Ⅱ+Losartan group was significantly lower than that in Ang Ⅱ group (P0.05). CONCLUSION Ang Ⅱ may induce intracellular calcium overload in RASMCs, which can be significantly reduced by Losartan. KEY WORDS: RASMCs; hypertension; angiotensin Ⅱ; Losartan; intracellular free calcium