苦豆子ISSR标记的遗传多样性分析.PDFVIP

中草药 Chinese Traditional and Herbal Drugs 第 44 卷 第 10 期 2013 年 5 月 ·1323 · 苦豆子 ISSR 标记的遗传多样性分析 1, 2 1* 1 1 阳 翠 ，刘 萍 ，刘姣蓉 ，郭 蒙 1. 宁夏大学 农学院，宁夏 银川 750021 2. 四川省植物工程研究院，四川 内江 641200 摘 要：目的 通过对产自宁夏、甘肃、青海、新疆和内蒙的苦豆子遗传多样性和亲缘关系的分析，为野生苦豆子资源的驯 化和保护等提供依据。方法 采用 ISSR 分子标记技术，对 22 个苦豆子居群的 DNA 进行扩增，对其扩增条带进行遗传多样 性分析，在所得遗传距离的基础上进行 UPGMA 聚类和主成分分析（PCA ），并绘制亲缘关系树状聚类图。结果 51 条 ISSR 引物共检测到 433 个位点，每条引物 5～12 个，平均 8.49 个；平均多态性位点百分率（PPB ）93.30% ；Nei’s 遗传多样性指 数（H ）和Shannon 信息指数（I ）分别为0.335 1、0.499 8 ；遗传距离变幅范围0.173 6～0.650 2 。结论 22 个苦豆子居群间 具有较高的遗传多样性，各居群间遗传距离与地理距离没有明显关系。 关键词：苦豆子；种质资源；ISSR 分子标记；遗传多样性；聚类分析 中图分类号：R282.12 文献标志码：A 文章编号：0253 - 2670(2013)10 - 1323 - 05 DOI: 10.7501/j.issn.0253-2670.2013.10.023 Genetic diversity analysis on Sophora alopecuroides based on ISSR markers 1, 2 1 1 1 YANG Cui , LIU Ping , LIU Jiao-rong , GUO Meng 1. College of Agronomy, Ningxia University, Yinchuan 750021, China 2. Sichuan Academy of Botanical Engineering, Neijiang 641200, China Abstract: Objective To analyze the genetic polymorphism and relationship of Sophora alopecuroides from Ningxia, Gansu, Qinghai, Xinjiang, and Inner Mongolian in China, and to offer some information for the domestication and protection of wild S. alopecuroides germplasms. Methods ISSR primers were used to analyze the genetic diversity of 22 populations of S. alopecuroides. Cluster analysis of the unweighted pair-group method with arithmetic average (UPGMA) and principal component analysis (PCA) were carried out based on the molecular data obtained, and a dendrogram was constructed. Results Totally 433 alleles were amplified using the 51 ISSR primers. The number of allels in per primer range was 5—12, with an average of 8.49. The average percentage of polymorphic loci (PPB) was 93.30%. The Nei’s gene diversity (H) and Shannon’s