两种方法检测梅毒螺旋体结果研究.docVIP

两种方法检测梅毒螺旋体结果研究【摘要】 目的 了解不同方法检测梅毒螺旋体结果的符合程度。方法 分别采集患者皮损拭净后轻刮或挤压至出渗液，用棉拭子取渗液作为PCR检测梅毒螺旋体标本，或棉拭子扦入男性尿道2～3 cm，女性宫颈管1～1．5 cm处取材作为PCR检测其他性病病原体标本，同时取患者血清作为RPR检测标本。结果 两种方法平行检测硬下疳、扁平湿疣及梅毒性斑丘疹患者的结果差异有统计学意义，其检出率也有较大的差异，PCR检测所得的结果均比RPR高。另外，STD患者中单纯梅毒螺旋体感染占构成比例80．68%(71/88)。合并感染其他STD病占19．32%(17/88)，其中合并感染淋球病6例，沙眼衣原体4例，解脲支原体3例，人乳头瘤2例，单纯疱疹病毒2例。结论 RPR和PCR虽是临床常用的检测方法，但是只是初筛试验，不能作为临床诊断梅毒的指标，需做TPPA进行确诊试验，才能诊断。 【关键词】梅毒螺旋体；性传播疾病；硬下疳；扁平湿疣；斑丘疹 Two methods for detecting treponema pallidum results DU Gui-nian， HUANG xian.Guangxi Guigang City People’s Hospital for Examination Branch，Guangxi Guigang，537100,China 【Abstract】 Objective Understand the different detection methods Treponema pallidum with the results of the degree.Methods Lesions were collected after Shicheng cheek or to squeeze out exudate，cotton swab from exudate as PCR Treponema pallidum specimens，or cotton swab stick into the male urethra 2-3 cm，a female cervica l-1．5 cm as PCR based STD pathogens other specimens while admission serum samples detected as RPR.Results 2 parallel detection method chancre hard，flat condyloma and syphilitic rash of patients have significant differences，and the detection rate is a greater difference，PCR results from the higher than RPR.In addition，the STD patients simply Treponema pallidum infection accounted for 80．68% mix(71/88).Other STD infection diseases accounted for 19．32%(17/88)，which combined the ball intentionally infected patients 6 cases，chlamydia trachomatis were 4 cases，the UU were 3 cases，people papilloma，2 cases of herpes simplex virus.Conclusion Although RPR and PCR is commonly used in clinical detection methods，but only screening test，not as clinical diagnosis of syphilis indicators to be done TPPA test for diagnosis，can be diagnosed. 【Key words】 Treponema pallidum；Sexually transmitted diseases；Hard chancre；Flat condyloma；Bihar 梅毒是由苍白梅毒螺旋体(TP)引起的一种性传播疾病。梅毒血清学诊断方法为非梅毒螺旋体血清试验和梅毒螺旋体血清试验,这两类血清学方法对梅毒的诊断具有特异性强、敏感性高等特点，但对早期梅毒螺旋体感染(4周前)的硬下疳患者敏感性较差，对神经梅毒和梅毒孕妇所生新生儿是否感染梅毒的