低分子肝素对脑缺血再灌注大鼠NF-κB、MPO活性影响.docVIP

低分子肝素对脑缺血再灌注大鼠NF-κB、MPO活性影响【摘要】 目的 探讨低分子肝素(Low molecular weight heparin,LMWH)对核转录因子-κB p65(NF-κB p65)和髓过氧化物酶(MPO)活性的影响。方法 将Wistar大鼠135只，随机分为假手术组、缺血再灌注组(IR组)、LMWH治疗组，线栓法建立局灶性脑缺血再灌注模型。IR组于再灌注前5 min尾静脉注射LMWH，1．5 mg/kg。采用免疫组化法和生化法观察各组缺血90 min再灌注3、6、12、24、48 h 点NF-κB p65表达、MPO活性变化，并进行脑含水量检测、TTC染色和HE染色观察脑梗死体积及病理形态学变化。结果 ①假手术组及IR组的未缺血侧大脑半球可见少量NF-κB p65表达于胞质, 再灌注后NF-κB p65表达发生核移位，于胞核表达增加，再灌注12 h达高峰；与IR组比较，LMWH治疗组能减少NF-κB p65的核移位(P 0．05)。结论 脑缺血再灌注损伤出现NF-κB活化和中性粒细胞浸润的炎性反应级联反应。LMWH对脑缺血再灌注损伤有保护作用，可能与减少NF-κB p65活化和减轻中性粒细胞浸润有关，且对凝血指标PT 和KPTT无明显影响。 【关键词】再灌注损伤；核转录因子-κB p65；髓过氧化物酶；低分子肝素；大鼠 Effect of Low molecular weight heparin on NF-κB and MPO Activity of cerebral ischemia reperfusion injury in rats LIU Kai-xiang, LI Hao,FEN Jun-lin,et al.Department of Neurology,the Affiliated Hospital of Guilin Medical College ,Guilin 541002,China 【Abstract】 Objective TO study effect of Low molecular weight heparin(LMWH) on NF-κB p65 and MPO of cerebral ischemia reperfusion injury in rats. Methods In this experiment, 135 Wistarrats were randomly divided into 3 groups, which were sham operated group, ischemia reperfusion group(IR group), LMWH treated group. The focal middle cerebral artery occlusion(MCAO) model was made by suture-occluded method. Rats were given LMWH by vena caudalis injection, 5 min before reperfusion.AfterMCAO following different durations of reperfusion,we investigated the expression of NF-κBp65 with using immunohistochemistry and investigated the MPO activityin the ischemic territory. The brain water content was examined.HE staining and TTC staining was also investigated. Results ①The translocation of NF-κBp65 from cytoplasm to nucleus increased significantly after reperfusion，and peaked at 12 h of reperfusion . Compared with IR group, LMWH treated group reduced expression ofNF-κBp65. ②MPO activitywasstarted to significantly increased at 12 h and peaked at 24 h of reperfusion in the ischemic territory. Compared with IR group,LMWH treated group reduced MPO activity. ③Co