泌尿系感染常见病原菌分布及耐药性研究.docVIP

泌尿系感染常见病原菌分布及耐药性研究【摘要】 目的 分析泌尿系感染病原菌的分布及耐药性特点,为临床选用抗菌药物提供依据。方法 对医院2007年10月至2009年 9月间,住院及门诊泌尿系感染患者尿培养分离出的286株病原菌进行鉴定和耐药分析。结果 在分离的286株病原菌中,革兰阴性菌占67.8%,革兰阳性菌占32.2%;革兰阴性菌以大肠埃希菌为主,革兰阳性菌以肠球菌属为主;产ESBLs大肠埃希菌检出率为34%,革兰阴性菌总的产酶率为26.3%;细菌对各种抗菌药物有不同程度的耐药率,表现为多重耐药。结论 分析泌尿系感染病原菌的分布及耐药性,对于控制耐药菌株传播、指导临床更加合理地应用抗菌药物具有十分重要的意义。? 【关键词】 泌尿系感染;病原菌;耐药性 Distribution of Pathogenic Bacteria in Urinary System Infection and Their Drug Resistance CHEN Dong-mei, ZHOU Feng-rong.Department of Medical Laboratory, The Peoples Hospital of LeiYang,LeiYang,HuNan, Leiyang 421800 China ? 【Abstract】 Objective To analyze the pathogenic bacterias distribution and their drug resistance in urinary system infection, and to provide the information for choosing suitable antibiotic. Methods The pathogens from the urine specimens were isolated and cultured from the patients in hospital from Oct 2007 to Sep 2009.The total 286 strains of pathogens were identified and the drug resistance was analyzed. Results In the 286 strains of bacteria,67.8% were Gram-negative and 32.2% were Grm-positive. Most of Gram-negative bacteria were Escherichia and most of Grm-positive bacteria were Enterococcus.34% of Escherichia produced ESBLs and the total rate of producing ESBLs was 26.3%. The bacteria showed different antibiotic resistance rate and multi-drug resistance.Conclusion It’s very important for making the clinical use of antibiotic more reasonable and controlling drug resistance strains transmission.? 【Key words】 Urinary System infeetion; Pathogenic bacteria; Resistance 作者单位:421800湖南省耒阳市人民医院检验科 泌尿系感染为医院内最为常见的感染之一[1],发病率仅次于呼吸系统感染。随着抗菌药物、免疫抑制剂、激素及介入诊断和治疗措施的增多,泌尿系感染发生率明显增加。越来越多的细菌出现了耐药性,从而增加了临床对泌尿系感染患者的治疗难度。为了解我院泌尿系感染患者的病原菌的分布及耐药性特点,我们对2007年10月至2009年 9月间,住院及门诊泌尿系感染患者尿培养分离出的286株病原菌进行鉴定和耐药分析,现将结果报告如下。 1 资料与方法 1.1 标本来源 2007年10月至2009年 9月间,住院患者及门诊患者尿液标本中分离得到,男95例,女191例。凡有尿频、尿急、尿痛等膀胱刺激症状,发热、肾区不适、排尿困难或无膀胱刺激症状等指征患者,在临床医师指导下,清洗、消毒外阴,采集晨起第一次中段尿液,置灭菌容器中立即送检,连续3次。 1.2 细菌培养鉴定及药敏 严格按《全国临床检验操作规程》进行操作,所有基础培养基