正交试验法优选金樱叶总黄酮提取工艺.docVIP

正交试验法优选金樱叶总黄酮提取工艺【摘要】 目的 优选金樱叶总黄酮的提取工艺,为金樱叶进一步的研究打下一定的基础。方法 采用L?9 (3?4)正交试验,以金樱叶总黄酮的含量为综合指标考察乙醇浓度、乙醇用量、提取时间和超声功率4个因素对提取效果的影响。结果 最佳提取工艺为40倍量50%的乙醇,28KHz、90W超声60 min,总黄酮得率为11.64%, RSD%为1.97%。结论 该工艺合理,稳定可行,可为金樱叶总黄酮提取工艺的确定提供实验依据。? 【关键词】 正交试验;金樱叶;总黄酮;提取工艺?? OptimumExtracting Tcchnology for Total Flavonoidsfrom Cherokee Rose’s Leaves by Orthogonal Test CHEN Ying-wen,CHEN Mu-shui.First hospital affilated to Guangzhou medical university.Guangzhou 510000,China ? 【Abstract】 Objective To optimize the extraction technology for total flavonoids from Cherokee Roses Leaves, and give reference for development and utilization of total ftavonoids.Methods The process was studied by orthogonal design L?9 (3?4) with the extraction rate of total flavonoids and paste- forming rate as comprehensive indexand researched the effect of alcohol amountalcohol dcnsity,extraction duration and the extraction frequency on extraction rate.Results The optimum process as followsthe crude drug was extracted by the 50% alcohol density40 times alcohol and 28KHz for 60 min. Conclusion This technology is rcasonable and feasible,which can be used as experimental basis for extraction technology for total ftavonoids.? 【Key words】 orthogonal test, Cherokee Rose’s Leaves,total flavonoids,extraction technology 金樱叶为蔷薇科植物金樱子Rosa laevigata Michx的嫩叶,味辣,性平。清热解毒、活血止血、止带、主痈肿疔疮、烫伤、痢疾、闭经、崩漏、带下、创伤出血[1]。金樱叶中含有多种有效成分,黄酮类如原矢车菊素(procyanidin)B-3、芹菜素等;鞣质类如蛇含鞣质(potentillin)、金樱子鞣质(laevigatin)C等。其中黄酮类成分具有体外抗肿瘤作用[2,3],因此本实验采用4因素3水平等正交试验L?9(3?4),选择总黄酮含量为综合考查指标,优化金樱叶的提取工艺,为开发新的抗癌药物提供理论参考。? 1 仪器与试药? 1.1 仪器 超声仪:KQ-3200VDB型双频数控超声波清洗器(昆山市舒美),紫外分光光度计:UV-1102紫外/可见分光光度计(上海天美);分析天平:SH 200E型电子天平,粉碎机:DFY-400,400 g摇摆式高速中药粉碎机(温岭市林大机械)。? 1.2 试药 材料:金樱叶来源于蔷薇科植物金樱子Rosa laevigata Michx的嫩叶,本实验采摘自中山市。试剂:芦丁对照品(中国药品生物制品检定所);硝酸铝、亚硝酸钠、氢氧化钠、乙醇为分析纯。? 2 方法与结果? 2.1 因素及水平考察 为系统考察乙醇超声提取金樱叶总黄酮的工艺参数,根据文献资料及实际情况选择乙醇浓度、超声时间、固液比和超声功率作为考察因素,以测得样品中总黄酮含量为考察指标,选用L?9 (3?4) 正交表实验,选出因素水平(见表1)。? 2.2 金樱叶的预处理 将采摘的金樱叶去除大部分枝梗,取叶片,晾干后用粉碎机粉碎成细