hela 细胞端粒d na 断裂损伤3 - current zoology.pdf

动物学报 　49 (6) :873～877 , 2003 A cta Zoologica S inica 　　　　　 　 Hela 细胞端粒 D NA 断裂损伤 ① ① ② ② ①② 阳　芳 　杨洁萍 　李清焕 　邵　兰 　谭　铮 ( ①武汉大学生命科学学院 , 武汉 430072) ( ②中国科学院动物研究所 , 北京 100080) Strand breaks in telomeres in Hela cells ① ① ② ② ①② YAN G Fang 　YAN G JiePing 　L I QingHuan 　SHAO Lan 　TAN Zheng ( ) ① College of L if e Sciences , W uhan U niversity , W uhan 　430072 , Chi na ( ) ② Instit ute of Zoology , Chi nese Academy of Sciences , Beiji n g 　100080 , Chi na Abstract 　Telomeres are the repetitive Grich DNA sequences at the end of chromosomes and shorten at each round of cell division. Besides the incomplete DNA synthesis , single and double DNA strand breaks , if not repaired , also contribute to the telomere shortening. To assess the frequency of strand breaks in proliferating Hela cells , telomere fragments were re leased by alkaline denaturing and electrophoresis from cells embedded in agarose , blotted onto membrane , and detected by probe specific to telomere sequence. The quantity of telomere fragments released was estimated to be less than 0. 4 % of the total telomere content , which corresponded to less than one break per cell. Since the mean length of the terminal re striction fragments of the cells was about 7 kbp , the fragments detected would lead to less than