复合酶法提取仿栗籽蛋白的工艺优化.PDFVIP

※工艺技术 食品科学 2012, Vol.33, No.20 27 1,2 2 2,3 2 2,3 2 麻成金 ，黄 伟 ，黄 群 ，冯 磊 ，吴丽雅 ，苏 超 (1.吉首大学 林产化工工程湖南省重点实验室，湖南 张家界 427000 ；2.吉首大学食品科学研究所，湖南 吉首 416000 ； 3.吉首大学 植物保护与利用湖南省高校重点实验室，湖南 吉首 416000) 摘 要：以仿栗籽为原料，选择多种酶对其蛋白质进行提取，筛选出纤维素酶与木瓜蛋白酶进行复合酶解试验。 以蛋白提取率为指标，在单因素试验的基础上，采用二次通用旋转组合设计对复合酶提取仿栗籽蛋白工艺进行优 化。结果表明：复合酶提取仿栗籽蛋白优化工艺条件为料液比1:25、总加酶量370U/g 、以纤维素酶与木瓜蛋白酶 的加酶量配比6:4 同时加入、酶解温度57℃、酶解时间80min、酶解pH7.6 。在此优化条件下，仿栗籽蛋白提取率为 74.91% 。 仿栗籽蛋白；提取；复合酶解；二次通用旋转组合设计 Optimization of Dual-Enzymatic Hydrolysis for Protein Extraction from Sloanea hemsleyana Seeds 1,2 2 2,3 2 2,3 2 MA Cheng-jin ，HUANG Wei ，HUANG Qun ，FENG Lei ，WU Li-ya ，SU Chao (1. Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, China ；2. Institute of Food Science, Jishou University, Jishou 416000, China ； 3. Key Laboratory of Plant Resources Conservation and Utilization of Hunan Province, Jishou University, Jishou 416000, China) Abstract ：Dual-enzymatic hydrolysis of Sloanea hemsleyana seeds with cellulase and papain was the best way for protein extraction as demonstrated by a comparison of the hydrolysis effi ciencies of 6 different enzymes on Sloanea hemsleyana seeds. The effects of simultaneous or stepwise hydrolysis, cellulase/papain ratio, solid/solvent ratio, total enzyme dosage, temperature, hydrolysis time and pH on protein yield were investigated using one-factor-at-a-time design. Further, total enzyme dosage, temperature, hydrolysis time and pH were optimized using quadr