镇痛灵在糖尿病防治中作用机制.docVIP

镇痛灵在糖尿病防治中作用机制【摘要】 目的 研究镇痛灵对Wistar糖尿病大鼠的防治作用机制。方法 3~5个月龄Wistar鼠75只，雌雄不限，随机分为糖尿病组60只，尾静脉推注1．5%四氧嘧啶50 mg/kg;正常对照组15只，尾静脉注射等体积生理盐水。72 h后，以血糖值大于16．67 mmol/L为造模成功。将糖尿病大鼠随机分为4组：①模型组；②3种不同剂量镇痛灵治疗组。第5周测血糖后乙醚麻醉下断头取血，分离血清测放免、生化指标。结果 ①1型糖尿病模型制备成功;②糖尿病大鼠与正常对照组比较,血清胰岛素(Ins)、C-肽、T-AOC明显降低，差异有高度显著性意义(P0．05)。结论 镇痛灵对糖尿病有防治作用，并在一定剂量范围内呈量效依赖关系。? 【关键词】大鼠；1型糖尿病；镇痛灵 Precaution and treatment effects on diabetes mellitus of super painkiller BAI Bing-sheng,LIU Jing-yao,WANG Chen.Department of Anesthesiology，the Second Hospital of Xiamen，Fujian 361011，China ? 【Abstract】 Objective To observe and explore the results relation of SuperPainkiller effects experimental the male wistar rats and precaution-treatment of diabetes.Methods 75 wistar rats at 4-6 months of age were divided into two groups at random:The group of diabetes mellitus were 60 rats，they were injected with Alloxan(50 mg/kg1．5%)(2)Control:15 rats were injected with the same dose of sodium chlorido injection.After 72 hours,we observed the blood glucose change，when blood glucose was higher than 16．67 mmol/L,the model was formed，the rats of diabetes mellituswere divided into 4 guoups.(1)The group of diabetes mellitus.(2)the group of diabetes mellituswas treated with super painkiller.After 5 weeks,We tookblood glucose.Got blood from vein，killed the rats，the other side were produced of micture，made serum out，measured dofa.Results (1)I type model of diabetes was formed.(2)Compared diabetes rats with normal group，serum Ins，C-P，T-AOC dropped sharply，the diversity wassignificantly.XO，blood glucose increasd(P0．05)。见表1。? 3 讨论? 3．1 关于1型糖尿病模型 糖尿病是由于持续性高血糖导致血管、神经、内分泌的一系列改变，诱发实验动物与临床患者出现多系统的并发症。? 本实验选用四氧嘧啶(alloxan)性糖尿病动物模型，类似人类1型糖尿病。一定量的四氧嘧啶注入大鼠尾静脉，可以成功复制1型糖尿病动物模型。因四氧嘧啶选择性破坏胰岛B细胞，使其合成胰岛素的功能发生障碍。胰岛细胞功能异常是糖尿病发病的基本环节。当B细胞不能适应环境变化以维持正常血糖水平时，血糖升高。持续高血糖可直接损伤B细胞，称为葡萄糖毒性。改善血糖状况，此毒性作用可有一定程度减轻或消失。? 对葡萄糖反应的敏感性是B细胞维持正常血糖的前提，但长期高血糖可抑制B细胞分泌胰岛素。当组织学改变后，即使恢复正常血糖也不能完全逆转已经发生的变化。? 四氧嘧啶易溶于水，其溶液不稳定，见光易分解成四氧嘧啶酸失效。应用时避光配制保存。研究表明Alloxan致