高脂饮食对大鼠血管内皮功能及腹主动脉形态学影响.docVIP

高脂饮食对大鼠血管内皮功能及腹主动脉形态学影响【摘要】 目的 观察高脂饮食对大鼠血管内皮功能和腹主动脉形态学的影响。方法 选用健康SD雄性大鼠16只，体质量150～180 g，随机分为正常对照组和高脂饮食组，每组8只。正常对照组用普通饲料喂养，高脂饮食组用高脂饲料喂养4周。观察高脂饮食对大鼠血浆血管紧张素Ⅱ(angiotensin Ⅱ, Ang Ⅱ)、血浆内皮素(endothelin, ET)和血清一氧化氮(nitrogen monoxide, NO)的影响，以及腹主动脉形态学变化。结果 4周后高脂饮食组血浆血管紧张素Ⅱ、血浆内皮素与正常对照组相比明显升高(P＜0．01)，血清NO与正常对照组相比明显降低(P＜0．01)；光学显微镜、透射电镜下见高脂饮食组腹主动脉壁结构异常。结论 高脂饮食可致大鼠血管内皮功能障碍和腹主动脉形态学改变。? 【关键词】高脂饮食；大鼠 ；内皮； 形态学?? Effect of high fat diet on rats of endothelial function and morphology of abdominal aorta ZHU Nai-xun, FENG Yi, ZHANG Li.Southeast Uniersity Subsidiary of the Chinese University Hospital,Nanjing 210009,China? 【Abstract】 Objective To observe the effect on rats of endothelial function and morphology of abdominal aorta． Methods 16 male SD rats were randomly divided into two groups: normal control group and high-fat group． The rats of high-fat group were fed with high fat diet while the control rats fed with normal pellet diet for a period of four weeks． At the ends of four weeks the indexes of plasma endothelin(ET), plasma angiotensin II (Ang II) and serum nitrogen monoxide (NO)were measured． Morphology of abdominal aorta were observed by light microscope and transmission electron microscope． Results At the end of four weeks plasma ET and plasma Ang II in the high-fat group were significantly higher than those in the control group(P＜0．01)． While serum NO were significantly lower than that in the control group(P＜0．01)． Vasculopathy of abdominal aorta were observed by light microscope and transmission electron microscope． Conclusion Endothelial dysfunction of rats and the change of morphology in abdominal aorta may be induced by high fat-feeding.? 【Key words】 High fat diet; Rats; Endothelium;Morphology 基金项目：苏州大学附属第一医院心血管病学“135工程”重点学科开放课题资助项目（项目编号：K0601）? 作者单位：210009东南大学附属中大医院心脏科(朱乃训 冯毅)；江苏省江都市人民医院心脏科(朱乃训)；上海市奉贤区中心医院心脏科(朱乃训 张立) 随着人们生活水平不断提高和生活方式以及饮食结构的改变，冠心病成为严重威胁人类健康的主要疾病之一，发病率有逐年增加的趋势。其主要的致病性危险因素有：脂质代谢紊乱、高血压、2型糖尿病、高胰岛素血症、向心性肥胖等。血管内皮损伤及功能障碍是动脉粥样硬化的始动因素。本