几种耐热戴氏霉对秸秆的降解效果 - 微生物所期刊联合编辑部.pdf

微生物学通报 Jul. 20, 2015, 42(7): 1279−1286 Microbiology China © 2015 by Institute of Microbiology, CAS tongbao@ DOI: 10.13344/j.microbiol.china.140661 研究报告 几种耐热戴氏霉对秸秆的降解效果 王垚 罗韵 梁宗琦 韩燕峰* (贵州大学 真菌资源研究所 贵州 贵阳 550025) 摘 要：【目的】测定6 株戴氏霉(Taifanglania)的生长温度特性及其对秸秆的降解效果。【方法】 通过定时测定不同培养温度下的菌落直径绘制6 株戴氏霉菌株的生长曲线；采用苯胺蓝法、愈创 木酚法和木质素磺酸钙降解试验测定其木质素降解能力；用羧甲基纤维素钠水解圈测定法和胞外 酶活测定法判定其对纤维素的降解能力；以失重法和范氏洗涤剂法检测其对水稻秸秆的降解效 果。【结果】所试的耐热戴氏霉菌株均能耐受50 °C 的高温，并能产生纤维素酶，但不同菌株产 生的木质素降解酶有所差异；均具有降解秸秆的能力，其中合川戴氏霉(T. hechuanensis) H08.1 菌株降解能力最强，其次是灰戴氏霉(T. cinerea) H57.1 菌株，其秸秆降解率分别为50.2%和42.2% 。 【结论】合川戴氏霉H08.1 菌株和灰戴氏霉H57.1 菌株在秸秆的降解利用上具有潜在开发价值。 关键词：耐热真菌，戴氏霉，秸秆降解，木质素降解酶，纤维素酶 Effect of several thermotolerant Taifanglania species on straw degradation * WANG Yao LUO Yun LIANG Zong-Qi HAN Yan-Feng (Institute of Fungus Resources, Guizhou University, Guiyang, Guizhou 550025, China) Abstract: [Objective] The objectives were to characterize growth temperature of six Taifanglania strains and to determine the effect on straw degradation. [Methods] Temperature-growth of six Taifanglania strains was monitored by colony diameters at different temperatures. The aniline blue method and guaiacol method combined with degradation of calcium lignosulfonate were used to test their lignin-degrading capability. Hydrolysis spot diameter measurement method of CMC-Na and extracellular