有机酸对铅、镉植株危害的解毒作用研究 - 环境科学学报.pdf

第 20 卷第 4 期 环 　境 　科 　学 　学 　报 Vol . 20 ,No . 4 2000 年 7 月 AC TA SCIEN T IA E CIRCU M STAN T IA E J uly ,2000 ( ) 文章编号 2000 　　　中图分类号 :X172 23 1 　　　文献标识码 :A 有机酸对铅 、镉植株危害的解毒作用研究 (浙江大学华家池校区环境保护系 , 杭州 　3 10029) 陈英旭 , 林 　琦 , 陆　芳 , 何云峰　 摘要 :通过培养试验研究了有机酸对铅 、镉的毒害影响 ,结果表明柠檬酸对铅 ,酒石酸对镉有较明显解毒作用. 用逐步提取法 研究萝 卜根叶内重金属存在的化学形态 ,有机酸处理并不影响各形态铅的优势顺序 ,但各形态铅的浓度或相对百分率发生 了变化 ,柠檬酸处理的萝 卜叶片 ,活性较低的盐酸提取态铅的百分率增加 ,活性较高的乙醇 、水 、氯化钠提取态下降; 镉在萝 卜根叶中均以氯化钠提取态为主 ,有机酸处理并不改变各形态镉的优势顺序 ,酒石酸处理中叶片氯化钠提取态镉较对照有 显著下降. 关键词 :柠檬酸 ;酒石酸 ;铅 ;镉 ;植株形态分析 Study on detoxication of organic acid to raddish under the stress of Pb and Cd ( CHEN Yingxu , L IN Qi , L U Fang , HE Yunfeng 　 Depart ment of Environmental Science , Zhejiang Universit y , Huajiachi , Hangzhou ,3 10029) Abstract :Effect of organic acid on raddish under t he stress of Pb and Cd was studied using hydroponic experiment . The result s showed t hat t he detoxication of citrate acid to Pb and tartaric acid to Cd was comparatively distinct . The former was probably due to improvement of transfer from root s to shoot s and t he latter , diversely , to inhibition of transfer from root s to shoot s , t hus decreased t he accumulation of Cd in shoot s. Wit h t he studies of heavy metal forms using sequential extraction , it showed t hat in t he root s of raddish , less activable HAc extractablePb was dominant and HCl , NaCl extractablePb followed. In t he shoot s , however , HCl ex tractablePb was dominant and NaCl extractablePb followed. Treat ment of organic acid had no effect on dominant sequence of differ ent Pb forms , but it caused t he variance of concentr